LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
CCNH / Cyclin H Antibody (C‑Terminus) LS‑C102865
Cyclin H antibody LS-C102865 is an unconjugated rabbit polyclonal antibody to Cyclin H (CCNH) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C102865-50
50 µg
$365
Description
Cyclin H antibody LS-C102865 is an unconjugated rabbit polyclonal antibody to Cyclin H (CCNH) from human, mouse, rat and other species. Validated for WB.
Target
Human CCNH / Cyclin H
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
CCNH / Cyclin H antibody was raised against synthetic peptide from C-Terminus of human CCNH (P51946, NP_001230). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Elephant, Bovine, Bat, Pig, Guinea pig (100%); Panda, Horse, Opossum (92%); Mouse, Rat, Hamster, Platypus (85%); Beetle (83%).
Blocking Peptide
LS-E10951 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C102865. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human CCNH / Cyclin H
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About CCNH / Cyclin H
P51946 NM_001239 NP_001230.1

Popular CCNH / Cyclin H Products

Immunofluorescence analysis of HeLa cells.
Species: Human
Applications: Immunofluorescence, Western blot, Immunoprecipitation
Antibody
Species: Human, Mouse
Applications: IHC, ICC, Western blot, Immunoprecipitation, Flow Cytometry
Anti-CCNH / Cyclin H antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Immunohistochemical analysis of Cyclin H staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Monkey, Mouse, Rat, Pig
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA

Publications (0)


Customer Reviews (0)


Images

Western blot

CCNH / Cyclin H antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CCNH / Cyclin H antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CCNH / Cyclin H antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CCNH / Cyclin H antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CCNH / Cyclin H antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CCNH / Cyclin H antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CCNH / Cyclin H antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CCNH / Cyclin H antibody Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/21/2018
Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy