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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CCNH / Cyclin H Antibody (aa269‑299) LS‑C168475
Cyclin H antibody LS-C168475 is an unconjugated rabbit polyclonal antibody to human Cyclin H (CCNH). Validated for Flow and WB.
Catalog
Size
Price
LS-C168475-400
400 µl
$305

Popular CCNH / Cyclin H Products

Immunofluorescence analysis of HeLa cells.
Species: Human
Applications: Western blot, Immunoprecipitation
Antibody
Species: Human, Rat, Dog, Pig
Applications: Immunoprecipitation
Antibody
Species: Human, Mouse
Applications: IHC, ICC, Western blot, Immunoprecipitation, Flow Cytometry
Anti-CCNH / Cyclin H antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6020 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Immunohistochemical analysis of Cyclin H staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Monkey, Mouse, Rat, Pig
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot

Product Description

Cyclin H antibody LS-C168475 is an unconjugated rabbit polyclonal antibody to human Cyclin H (CCNH). Validated for Flow and WB.
About CCNH / Cyclin H
CCNH / Cyclin H belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. P51946 NM_001239 NP_001230.1

CCNH Antibody, Cyclin H Antibody, p34 Antibody, p37 Antibody, CAK Antibody, CAK complex subunit Antibody, Cyclin-H Antibody, MO15-associated protein Antibody


Specifications

Target
Human CCNH / Cyclin H
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Blocking Peptide
LS-E609 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C168475. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa269-299
Specificity
This CCNH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 269-299 amino acids from the C-terminal region of human CCNH.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Western blot

CCNH Antibody western blot of HeLa cell line lysates (35 ug/lane). The CCNH antibody detected the CCNH protein (arrow).
CCNH Antibody western blot of HeLa cell line lysates (35 ug/lane). The CCNH antibody detected the CCNH protein (arrow).

Flow Cytometry

CCNH Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CCNH Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot

CCNH Antibody western blot of HeLa cell line lysates (35 ug/lane). The CCNH antibody detected the CCNH protein (arrow).
CCNH Antibody western blot of HeLa cell line lysates (35 ug/lane). The CCNH antibody detected the CCNH protein (arrow).

Flow Cytometry

CCNH Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CCNH Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot

CCNH Antibody western blot of HeLa cell line lysates (35 ug/lane). The CCNH antibody detected the CCNH protein (arrow).
CCNH Antibody western blot of HeLa cell line lysates (35 ug/lane). The CCNH antibody detected the CCNH protein (arrow).

Flow Cytometry

CCNH Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CCNH Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot

CCNH Antibody western blot of HeLa cell line lysates (35 ug/lane). The CCNH antibody detected the CCNH protein (arrow).
CCNH Antibody western blot of HeLa cell line lysates (35 ug/lane). The CCNH antibody detected the CCNH protein (arrow).

Flow Cytometry

CCNH Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CCNH Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 8/21/2018

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