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CCND1 / Cyclin D1 Antibody LS-C18809

Western blot analysis is shown using Anti-Cyclin D1 antibody to detect Human Cyclin D1 present in asynchronous HN30 cell lysates. HN30 cells, are from head and neck cancer cells that over express cyclin B1 and D1. Comparison to a molecular weight marker indicates a band of ~34 kD corresponding to the expected molecular weight for the protein (arrowhead). The blot was incubated with a 1:500 dilution of the antibody at room temperature. Detection occurred using a 1:10000 of HRP conjugated Go.

CCND1 / Cyclin D1 Antibody LS-C18809

Antibody:
Rabbit Polyclonal to Human CCND1 / Cyclin D1
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C18809-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human CCND1 / Cyclin D1
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Cyclin D1 antibody LS-C18809 is an unconjugated rabbit polyclonal antibody to human Cyclin D1 (CCND1). Validated for ELISA and WB.

Target
Human CCND1 / Cyclin D1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:1000)
  • ELISA (1:2000 - 1:10000)
Failed Applications
  • IHC - Paraffin
Specificity
Full length synthetic polypeptide corresponding to the human gene sequence.
Usage
This antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 34 kD in size corresponding to Cyclin D1 by western blotting in the appropriate cell lysate or extract. Anti-Cyclin D1 is suitable for the detection by immunoblot of human, rat and mouse Cyclin D1.
Presentation
0.02M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide.
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCND1 / Cyclin D1
P24385 NM_053056 NP_444284.1

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