Products
Research Areas
COVID-19
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
206-374-1102
Fax:
206-577-4565
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, Proforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C22518-200 200 µg $673 
Antibody

Polyclonal Rabbit anti‑Human CCND1 / Cyclin D1 Antibody (aa285‑295, WB) LS‑C22518

Polyclonal Rabbit anti‑Human CCND1 / Cyclin D1 Antibody (aa285‑295, WB) LS‑C22518

Antibody:
CCND1 / Cyclin D1 Rabbit anti-Human Polyclonal (aa285-295) Antibody
Application:
ICC, WB, IP, Func
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Hamster
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$673
LS-C22518-200
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CCND1 / Cyclin D1 Rabbit anti-Human Polyclonal (aa285-295) Antibody
Application:
ICC, WB, IP, Func
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Hamster
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Cyclin D1 antibody LS-C22518 is an unconjugated rabbit polyclonal antibody to Cyclin D1 (CCND1) (aa285-295) from human. It is reactive with human, mouse, rat and other species. Validated for Func, ICC, IP and WB.
Target
Human CCND1 / Cyclin D1
Synonyms
CCND1 | B-cell lymphoma 1 protein | BCL-1 oncogene | B-cell CLL/lymphoma 1 | BCL-1 | Cyclin D1 | G1/S-specific cyclin-D1 | U21B31 | PRAD1 | BCL1 | D11S287E | PRAD1 oncogene
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Hamster (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to the 11 C-Terminus amino acids (residues 285-295; sequence: CTPTDVRDVDI) of human Cyclin D1. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Bat (100%); Elephant, Panda, Bovine, Dog, Horse, Turkey, Chicken, Platypus, Lizard, Xenopus, Salmon, Pufferfish (91%); Opossum, Stickleback, Zebrafish (82%).
Epitope
aa285-295
Specificity
Recognizes p36 Cyclin D1 and cross-reacts with p34 Cyclin D2, which has a highly homologous epitope. No cross-reactivity with Cyclin A or Cyclin B. Shows reactivity with a 28kD band that can competed away by pre-incubating antibody with the peptide immunogen. Also detect a nonspecific band at 21kD. Species cross-reactivity: mouse and rat.
Applications
  • ICC
  • Western blot
  • Immunoprecipitation
  • Functional Assay
Usage
Western Blot Analysis 2: At 0.5-2 ug/ml, this antibody detected Cyclin D in a RIPA lysates of human A431 cells, mouse 3T3 fibroblasts. Positive Antigen Control: EGF-stimulated A431 cell lysate. Use 20 ug per lane. Immunoprecipitation: 5 ug of antibody immunoprecipitated Cyclin D from a RIPA lysate of human A431 cells. An H1 kinase assay can be performed with the immunoprecipitate, as reported previously. Immunocytochemistry: At 10 ug/ml, a previous lot of antibody detected Cyclin D in cells fixed with 4% paraformaldehyde/0.1% Triton X-100. Western Blot Analysis EGF-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Cyclin D (2 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCND1 / Cyclin D1
P24385 NM_053056 NP_444284.1

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022