Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C801027-100 100 µl (1 mg/ml) $379 
LS-C801027-200 200 µl (1 mg/ml) $421 
CCNB1 / Cyclin B1 Antibody - Staining NIH-3T3 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
CCNB1 / Cyclin B1 Antibody - Western blot analysis of Phospho-Cyclin B1 (Ser147) expression in various lysates
CCNB1 / Cyclin B1 Antibody - Western blot analysis of Cyclin B1 phosphorylation expression in UV treated Jurkat whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
CCNB1 / Cyclin B1 Antibody - Staining NIH-3T3 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
CCNB1 / Cyclin B1 Antibody - Western blot analysis of Phospho-Cyclin B1 (Ser147) expression in various lysates
CCNB1 / Cyclin B1 Antibody - Western blot analysis of Cyclin B1 phosphorylation expression in UV treated Jurkat whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
1 of 3
2 of 3
3 of 3

Polyclonal Rabbit anti‑Human CCNB1 / Cyclin B1 Antibody (phospho‑Ser147, IHC, IF, WB) LS‑C801027

Polyclonal Rabbit anti‑Human CCNB1 / Cyclin B1 Antibody (phospho‑Ser147, IHC, IF, WB) LS‑C801027

Antibody:
CCNB1 / Cyclin B1 Rabbit anti-Human Polyclonal (pSer147) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801027-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CCNB1 / Cyclin B1 Rabbit anti-Human Polyclonal (pSer147) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Cyclin B1 antibody LS-C801027 is an unconjugated rabbit polyclonal antibody to Cyclin B1 (CCNB1) (pSer147) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human CCNB1 / Cyclin B1
Synonyms
CCNB1 | CCNB | Cyclin B1 | G2/mitotic-specific cyclin-B1 | G2/mitotic-specific cyclin B1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Cyclin B1 around the phosphorylation site of Serine 147.
Epitope
pSer147
Specificity
Phospho-Cyclin B1 (Ser147) Antibody detects endogenous levels of Cyclin B1 only when phosphorylated at Serine 147.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCNB1 / Cyclin B1
P14635 NM_031966 NP_114172.1

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/23/2024