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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CCNB1 / Cyclin B1 Antibody (Biotin) LS-C525326

Cyclin B1 antibody LS-C525326 is a biotin-conjugated mouse monoclonal antibody to Cyclin B1 (CCNB1) from human, mouse, rat and other species. Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C525326-100
100 µl
$860
Description
Cyclin B1 antibody LS-C525326 is a biotin-conjugated mouse monoclonal antibody to Cyclin B1 (CCNB1) from human, mouse, rat and other species. Validated for Flow, IHC and WB.
Target
Hamster CCNB1 / Cyclin B1
Host
Mouse
Reactivity
Hamster, Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Biotin. Also available conjugated with AP, APC, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • Western blot
  • Flow Cytometry
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Hamster cyclin B1 protein.
Specificity
Recognizes Cyclin B1 at 62kD. Species Reactivity: Human, Mouse, Rat and Hamster.
Presentation
PBS, pH 7.2, No preservatives added
Storage
Store at 4°C.
Restrictions
For research use only.
About CCNB1 / Cyclin B1
P14635 NM_031966 NP_114172.1

Popular CCNB1 / Cyclin B1 Products

Anti-Cyclin B1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Species: Hamster, Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Cyclin B1 Antibody. The picture on the right is blocked with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human, Mouse, Rat, Hamster
Applications: IHC, IHC - Paraffin, Immunofluorescence, Immunoprecipitation
Antibody
Species: Human
Applications: Western blot, Immunoprecipitation
Western blot analysis using Anti-Cyclin B1 antibody shows detection of human Cyclin B1 present in asynchronous HN30 cell lysates. HN30 cells are from head and neck cancer tumors that over express cyclin B1 and D1. Comparison to a molecular weight marker indicates a band of ~62 kD corresponding to the expected molecular weight for the protein (arrowhead). The blot was incubated with a 1:500 dilution of the antibody for 1 h at room temperature. Detection occurred using a 1:10000 of HRP conjugated Goat-a-Rabbit IgG LS-C60865 and chemiluminescence reagent with a 1-min exposure time. Other detection systems will yield similar results.
Species: Human
Applications: Western blot, Immunoprecipitation, ELISA

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Requested From: United States
Date Requested: 4/25/2019
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