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Catalog Number Size Price
LS-C407638-10 10 µg $318 
LS-C407638-100 100 µg $470 
CCNA2 / Cyclin A2 Antibody - Cyclin A2 antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.
CCNA2 / Cyclin A2 Antibody - IF analysis of Cyclin A2 using anti-Cyclin A2 antibody Cyclin A2 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-Cyclin A2 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CCNA2 / Cyclin A2 Antibody - Cyclin A2 antibody Western blot. All lanes: Anti Cyclin A2 at 0.5 ug/ml. Lane 1: Rat Skeletal Muscle Tissue Lysate at 50 ug. Lane 2: HELA Whole Cell Lysate at 40 ug. Lane 3: COLO320 Whole Cell Lysate at 40 ug. Lane 4: HEPG2 Whole Cell Lysate at 40 ug. Lane 5: MCF-7 Whole Cell Lysate at 40 ug. Predicted band size: 49 kD. Observed band size: 49 kD.
CCNA2 / Cyclin A2 Antibody - Flow Cytometry analysis of U20S cells using anti-Cyclin A2 antibody. Overlay histogram showing U20S cells stained with anti-Cyclin A2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cyclin A2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CCNA2 / Cyclin A2 Antibody - Cyclin A2 antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.
CCNA2 / Cyclin A2 Antibody - IF analysis of Cyclin A2 using anti-Cyclin A2 antibody Cyclin A2 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-Cyclin A2 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CCNA2 / Cyclin A2 Antibody - Cyclin A2 antibody Western blot. All lanes: Anti Cyclin A2 at 0.5 ug/ml. Lane 1: Rat Skeletal Muscle Tissue Lysate at 50 ug. Lane 2: HELA Whole Cell Lysate at 40 ug. Lane 3: COLO320 Whole Cell Lysate at 40 ug. Lane 4: HEPG2 Whole Cell Lysate at 40 ug. Lane 5: MCF-7 Whole Cell Lysate at 40 ug. Predicted band size: 49 kD. Observed band size: 49 kD.
CCNA2 / Cyclin A2 Antibody - Flow Cytometry analysis of U20S cells using anti-Cyclin A2 antibody. Overlay histogram showing U20S cells stained with anti-Cyclin A2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cyclin A2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human CCNA2 / Cyclin A2 Antibody (aa10‑168, IHC, WB) LS‑C407638

Polyclonal Rabbit anti‑Human CCNA2 / Cyclin A2 Antibody (aa10‑168, IHC, WB) LS‑C407638

Antibody:
CCNA2 / Cyclin A2 Rabbit anti-Human Polyclonal (aa10-168) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407638-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CCNA2 / Cyclin A2 Rabbit anti-Human Polyclonal (aa10-168) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Rat, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
Cyclin A2 antibody LS-C407638 is an unconjugated rabbit polyclonal antibody to Cyclin A2 (CCNA2) (aa10-168) from human. It is reactive with human, rat and dog. Validated for IHC and WB.
Target
Human CCNA2 / Cyclin A2
Synonyms
CCNA2 | Cyclin-A2 | Cyclin A2 | CCN1 | CCNA | Cyclin-A | G2/mitotic-specific cyclin a
Host
Rabbit
Reactivity
Human, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human Cyclin A2 recombinant protein (Position: A10-K168). Human Cyclin A2 shares 74.5% amino acid (aa) sequence identity with mouse Cyclin A2.
Epitope
aa10-168
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCNA2 / Cyclin A2
P20248 NM_001237 NP_001228.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/24/2024