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Catalog Number Size Price
LS-C38737-100 100 µg $930 
Antibody

Polyclonal Sheep anti‑Cat CCL5 / RANTES Antibody (WB) LS‑C38737

Polyclonal Sheep anti‑Cat CCL5 / RANTES Antibody (WB) LS‑C38737

Antibody:
CCL5 / RANTES Sheep anti-Cat Polyclonal Antibody
Application:
WB, ELISA, Func, Neut
Reactivity:
Cat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$930
LS-C38737-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CCL5 / RANTES Sheep anti-Cat Polyclonal Antibody
Application:
WB, ELISA, Func, Neut
Reactivity:
Cat
Format:
Unconjugated, Unmodified

Specifications

Description
RANTES antibody LS-C38737 is an unconjugated sheep polyclonal antibody to cat RANTES (CCL5). Validated for ELISA, Func, Neut and WB.
Target
Cat CCL5 / RANTES
Synonyms
CCL5 | Chemokine (C-C motif) ligand 5 | D17S136E | EoCP | T-cell specific protein p288 | SCYA5 | SISd | T cell-specific protein P228 | Beta-chemokine RANTES | C-C motif chemokine 5 | RANTES | SIS-delta | Small-inducible cytokine A5 | T-cell-specific protein RANTES | TCP228
Host
Sheep
Reactivity
Cat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived rfeRANTES.
Specificity
Selected for its ability to neutralize feline RANTES bioactivity.
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 1 µg/ml)
  • Functional Assay
  • Neutralization
Usage
Suitable for use in ELISA, Western Blot, Neutralization. Neutralization of Feline CCL5 Bioactivity: The exact concentration of antibody required to neutralize rfeRANTES activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of anti-feline RANTES antibody was determined to be approximately 0.5-2.5 ug/ml in the presence of 80 ng/mL of rfeRANTES, using transfected BaF/3 cells in a chemotaxis assay. The specific conditions are described in the figure legends. Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect feline RANTES. The detection limit for rfeRANTES is approximately 1 ng/lane under non-reducing and reducing conditions. Direct ELISA: 0.5-1.0 ug/ml with the appropriate secondary reagents to detect feline RANTES. The detection limit for rfeRANTES is approximately 0.5 ng/well. In this format, this antibody shows less than 5% cross-reactivity with rcrRANTES, rhRANTES, and rmRANTES.
Presentation
Lyophilized
Reconstitution
Reconstitute in deionized water
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCL5 / RANTES
P13501 NM_002985 NP_002976.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/16/2024