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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C153848-100 100 µg (1 mg/ml) $425 
CCL4 / MIP-1 Beta Antibody - Western blot of protein-A purified Anti-MIP-1 (CCL4) antibody shows detection of recombinant swine MIP-1 (CCL4) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 4 g/mL primary antibody overnight at 4C. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG (p/n 611-132-122) at 1:20000 for 45 min at room temperature.

Polyclonal CCL4 / MIP‑1 Beta Antibody (WB) LS‑C153848

Polyclonal CCL4 / MIP‑1 Beta Antibody (WB) LS‑C153848

Antibody:
Rabbit Polyclonal to Pig CCL4 / MIP-1 Beta
Application:
WB, ELISA
Reactivity:
Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C153848-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Pig CCL4 / MIP-1 Beta
Application:
WB, ELISA
Reactivity:
Pig
Format:
Unconjugated, Unmodified

Specifications

Description
MIP-1 Beta antibody LS-C153848 is an unconjugated rabbit polyclonal antibody to pig MIP-1 Beta (CCL4 ). Validated for ELISA and WB.
Target
Pig CCL4 / MIP-1 Beta
Synonyms
CCL4 | ACT2 | Act-2 | AT744.1 | C-C motif chemokine 4 | Chemokine (C-C motif) ligand 4 | CC chemokine ligand 4 | HC21 | LAG1 | MIP1B | MIP1B1 | LAG-1 | SCYA2 | Secreted protein G-26 | SIS-gamma | MIP-1-beta | MIP-1-beta(1-69) | Small-inducible cytokine A4 | T-cell activation protein 2 | G-26 | Lymphocyte-activation gene 1 | PAT 744 | Protein H400 | SCYA4
Host
Rabbit
Reactivity
Pig (tested or 100% immunogen sequence identity)
Predicted
Horse, Panda (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Applications
  • Western blot (1:500)
  • ELISA (1:5000)
Immunogen
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant protein raised in yeast, corresponding to the 69 amino acids of the mature swine CCL4/MIP1ß protein.
Specificity
This antibody is specific for swine CCL4 protein. A BLAST analysis was used to suggest cross-reactivity with CCL4 from swine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against horse and panda CCL4 based on 90% homology. Cross-reactivity with CCL4 from other sources has not been determined. The swine CCL4 sequence is also 86% homologous to swine CCL3L1.
Usage
This protein-A purified antibody is suitable for ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 7.8 kD in size corresponding to swine CCL4 protein by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCL4 / MIP-1 Beta
P13236 NM_002984 NP_002975.1

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Requested From: United States
Date Requested: 2/17/2020