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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CCL4 / MIP‑1 Beta Antibody LS‑C153848
MIP-1 Beta antibody LS-C153848 is an unconjugated rabbit polyclonal antibody to pig MIP-1 Beta (CCL4). Validated for ELISA and WB.
Catalog
Size
Price
LS-C153848-100
100 µg
$425
Description
MIP-1 Beta antibody LS-C153848 is an unconjugated rabbit polyclonal antibody to pig MIP-1 Beta (CCL4). Validated for ELISA and WB.
Target
Pig CCL4 / MIP-1 Beta
Host
Rabbit
Reactivity
Pig (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:500)
  • ELISA
Immunogen
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant protein produced in yeast, corresponding to the 69 amino acids of the mature swine CCL4/MIP1 protein.
Specificity
This antibody is specific for swine CCL4 protein. A BLAST analysis was used to suggest cross-reactivity with CCL4 from swine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against horse and panda CCL4 based on 98% homology, with bovine, dog, and rabbit based on 97% homology, macaque and feline based on 95%, with human and chimpanzee based on 94%, and with rat and opossum CCL4 based on 91% homology. Cross-reactivity with CCL4 from other sources has not been determined. The swine CCL4 sequence is also 86% homologous to swine CCL3L1.
Usage
This protein-A purified antibody is suitable for ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 7.8 kD in size corresponding to swine CCL4 protein by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
100 µl deionized water
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
About CCL4 / MIP-1 Beta
P13236 NM_002984 NP_002975.1

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Images

Western blot

Western blot of protein-A purified Anti-MIP-1 (CCL4) antibody shows detection of recombinant swine MIP-1 (CCL4) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 4 g/mL primary antibody overnight at 4C. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG (p/n 611-132-122) at 1:20000 for 45 min at room temperature.
Western blot of protein-A purified Anti-MIP-1 (CCL4) antibody shows detection of recombinant swine MIP-1 (CCL4) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 4 g/mL primary antibody overnight at 4C. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG (p/n 611-132-122) at 1:20000 for 45 min at room temperature.

Western blot

Western blot of protein-A purified Anti-MIP-1 (CCL4) antibody shows detection of recombinant swine MIP-1 (CCL4) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 4 g/mL primary antibody overnight at 4C. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG (p/n 611-132-122) at 1:20000 for 45 min at room temperature.
Western blot of protein-A purified Anti-MIP-1 (CCL4) antibody shows detection of recombinant swine MIP-1 (CCL4) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 4 g/mL primary antibody overnight at 4C. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG (p/n 611-132-122) at 1:20000 for 45 min at room temperature.

Western blot

Western blot of protein-A purified Anti-MIP-1 (CCL4) antibody shows detection of recombinant swine MIP-1 (CCL4) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 4 g/mL primary antibody overnight at 4C. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG (p/n 611-132-122) at 1:20000 for 45 min at room temperature.
Western blot of protein-A purified Anti-MIP-1 (CCL4) antibody shows detection of recombinant swine MIP-1 (CCL4) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 4 g/mL primary antibody overnight at 4C. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG (p/n 611-132-122) at 1:20000 for 45 min at room temperature.

Western blot

Western blot of protein-A purified Anti-MIP-1 (CCL4) antibody shows detection of recombinant swine MIP-1 (CCL4) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 4 g/mL primary antibody overnight at 4C. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG (p/n 611-132-122) at 1:20000 for 45 min at room temperature.
Western blot of protein-A purified Anti-MIP-1 (CCL4) antibody shows detection of recombinant swine MIP-1 (CCL4) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 4 g/mL primary antibody overnight at 4C. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG (p/n 611-132-122) at 1:20000 for 45 min at room temperature.

Requested From: United States
Date Requested: 11/16/2018
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