MIP-1 Beta antibody LS-C153848 is an unconjugated rabbit polyclonal antibody to pig MIP-1 Beta (CCL4). Validated for ELISA and WB.
Pig CCL4 / MIP-1 Beta
(tested or 100% immunogen sequence identity)
Protein A purified
- Western blot (1:500)
This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant protein produced in yeast, corresponding to the 69 amino acids of the mature swine CCL4/MIP1 protein.
This antibody is specific for swine CCL4 protein. A BLAST analysis was used to suggest cross-reactivity with CCL4 from swine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against horse and panda CCL4 based on 98% homology, with bovine, dog, and rabbit based on 97% homology, macaque and feline based on 95%, with human and chimpanzee based on 94%, and with rat and opossum CCL4 based on 91% homology. Cross-reactivity with CCL4 from other sources has not been determined. The swine CCL4 sequence is also 86% homologous to swine CCL3L1.
This protein-A purified antibody is suitable for ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 7.8 kD in size corresponding to swine CCL4 protein by western blotting in the appropriate cell lysate or extract.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.