Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
CCL4, ACT2, Act-2, AT744.1, C-C motif chemokine 4, Chemokine (C-C motif) ligand 4, CC chemokine ligand 4, HC21, LAG1, MIP1B, MIP1B1, LAG-1, SCYA2, Secreted protein G-26, SIS-gamma, MIP-1-beta, MIP-1-beta(1-69), Small-inducible cytokine A4, T-cell activation protein 2, G-26, Lymphocyte-activation gene 1, PAT 744, Protein H400, SCYA4
(tested or 100% immunogen sequence identity)
IHC (0.5 - 5 µg/ml)
Western blot (0.1 - 0.2 µg/ml)
ELISA (0.5 - 1 µg/ml)
Neutralization (0.3 - 1.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Sf 21-derived Recombinant, human Macrophage Inflammatory Protein 1 beta (MIP1 beta, CCL4).
Recognizes Macrophage Inflammatory Protein 1 beta (MIP1 beta). based on ELISA and Western Blot data, <10% cross-reactivity with mouse MIP-1b, human MIP-1b, human MCP-2 and <5% cross-reactivity with mouse MIP-1b. <1% cross-reactivity with other chemokines tested.
Suitable for use in neutralization of bioactivity, ELISA, Western Blot, and Immunohistochemistry. Neutralization: 0.3-1.5 ug/ml in the presence of 0.04 ug/ml of rhMIP-1b, using the BaF/3 hCCR5 transfected cell chemotaxis assay. In chemotaxis assays, this antibody will neutralize the biological activity of human MIP-1b and mouse MIP-1b at a 25-fold higher IgG concentration. Will not neutralize the biological activity of mouse MIP1b. ELISA: 0.5-1 ug/ml with the appropriate secondary reagents to detect human MIP-1b. The detection limit is ~0.3 ng/well. Western Blot: 0.1-0.2 ug/ml. The detection limit is 1.5-3.0 ng/lane, under non-reducing and reducing conditions. Immunohistochemistry: 0.5-5 ug/ml in cultured cells or tissue. sections.
PBS, pH 7.2, 40% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells.