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CCL4 / MIP-1 Beta Antibody LS-C8611

Antibody

CCL4 / MIP-1 Beta Antibody LS-C8611

Antibody:
Goat Polyclonal to Human CCL4 / MIP-1 Beta
Application:
IHC, WB, ELISA, Neut
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$785 
LS-C8611-100
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human CCL4 / MIP-1 Beta
Application:
IHC, WB, ELISA, Neut
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
MIP-1 Beta antibody LS-C8611 is an unconjugated goat polyclonal antibody to MIP-1 Beta (CCL4) from human and mouse. Validated for ELISA, IHC, Neut and WB.

Target
Human CCL4 / MIP-1 Beta
Host
Goat
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC (0.5 - 5 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 1 µg/ml)
  • Neutralization (0.3 - 1.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Sf 21-derived Recombinant, human Macrophage Inflammatory Protein 1 beta (MIP1 beta, CCL4).
Specificity
Recognizes Macrophage Inflammatory Protein 1 beta (MIP1 beta). based on ELISA and Western Blot data, <10% cross-reactivity with mouse MIP-1b, human MIP-1b, human MCP-2 and <5% cross-reactivity with mouse MIP-1b. <1% cross-reactivity with other chemokines tested.
Usage
Suitable for use in neutralization of bioactivity, ELISA, Western Blot, and Immunohistochemistry. Neutralization: 0.3-1.5 ug/ml in the presence of 0.04 ug/ml of rhMIP-1b, using the BaF/3 hCCR5 transfected cell chemotaxis assay. In chemotaxis assays, this antibody will neutralize the biological activity of human MIP-1b and mouse MIP-1b at a 25-fold higher IgG concentration. Will not neutralize the biological activity of mouse MIP1b. ELISA: 0.5-1 ug/ml with the appropriate secondary reagents to detect human MIP-1b. The detection limit is ~0.3 ng/well. Western Blot: 0.1-0.2 ug/ml. The detection limit is 1.5-3.0 ng/lane, under non-reducing and reducing conditions. Immunohistochemistry: 0.5-5 ug/ml in cultured cells or tissue. sections.
Presentation
PBS, pH 7.2, 40% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCL4 / MIP-1 Beta
P13236 NM_002984 NP_002975.1

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Reactivity: All species
Range: 12.35-1000 ng/ml
Reactivity: Human
Range: 24.69-18000 pg/ml
Reactivity: Human
Range: 46.88-3000 pg/ml
Reactivity: Monkey
Range: 0.156-10 ng/ml

Requested From: United States
Date Requested: 5/27/2019
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