Human CCL3 + CC3L1

Goat

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence C-EWVQKYVSDLELSA, from the C-Terminus of the protein sequence according to NP_002974.1; NP_066286.1.

C-Terminus

Please note this product is expected to recognize the products of 2 different genes which are almost identical.

• Peptide Enzyme-Linked Immunosorbent Assay (1:32000): Detection Limit

Western Blot: Preliminary experiments gave bands at approx 26+28kDa (doublet) and 40kDa in Human Bone Marrow and Spleen lysates after 0.1µg/ml antibody staining.The 26+28kDa doublet was also found in Human Lymph Node and in Tonsil lysates. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 10.2kDa according to NP_066286.1. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain these bands).

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

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