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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C154085-100 100 µg (1 mg/ml) $425 
CCL20 / MIP-3-Alpha Antibody - Anti-Human MIP-3a Antibody - Western Blot. Western blot of anti-Human MIP-3a antibody shows detection of a band ~26 kD in size corresponding to recombinant human MIP-3a (lane 1). The identity of the lower molecular weight band is unknown. Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (LS-C60865) secondary antibody diluted 1:40000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).

CCL20 / MIP‑3‑Alpha Antibody LS‑C154085

CCL20 / MIP‑3‑Alpha Antibody LS‑C154085

Antibody:
Rabbit Polyclonal to Human CCL20 / MIP-3-Alpha
Application:
WB, ELISA, Neut
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C154085-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human CCL20 / MIP-3-Alpha
Application:
WB, ELISA, Neut
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
MIP-3-Alpha antibody LS-C154085 is an unconjugated rabbit polyclonal antibody to human MIP-3-Alpha (CCL20 ). Validated for ELISA, Neut and WB.
Target
Human CCL20 / MIP-3-Alpha
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:2000)
  • ELISA
  • Neutralization (1:400)
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-3a protein.
Specificity
This Protein A purified antibody was heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human MIP-3a in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Usage
This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 11 kD in size corresponding to native human MIP-3a protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
Reconstitution
Reconstitute in deionized water
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCL20 / MIP-3-Alpha
P78556 NM_004591 NP_004582.1

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Requested From: United States
Date Requested: 11/19/2019