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Catalog Number Size Price
LS-C154085-100 100 µg (1 mg/ml) $567 
CCL20 / MIP-3-Alpha Antibody - Anti-Human MIP-3a Antibody - Western Blot. Western blot of anti-Human MIP-3a antibody shows detection of a band ~26 kD in size corresponding to recombinant human MIP-3a (lane 1). The identity of the lower molecular weight band is unknown. Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (LS-C60865) secondary antibody diluted 1:40000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).

Polyclonal Rabbit anti‑Human CCL20 / MIP‑3‑Alpha Antibody (WB) LS‑C154085

Polyclonal Rabbit anti‑Human CCL20 / MIP‑3‑Alpha Antibody (WB) LS‑C154085

Antibody:
CCL20 / MIP-3-Alpha Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C154085-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CCL20 / MIP-3-Alpha Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
MIP-3-Alpha antibody LS-C154085 is an unconjugated rabbit polyclonal antibody to human MIP-3-Alpha (CCL20). Validated for ELISA and WB.
Target
Human CCL20 / MIP-3-Alpha
Synonyms
CCL20 | Beta chemokine exodus-1 | Beta-chemokine exodus-1 | CKb4 | Exodus-1 | MIP-3a | MIP-3-alpha | LARC | ST38 | SCYA20 | C-C motif chemokine 20 | CC chemokine LARC | Mip-3alpha | MIP3A | Small-inducible cytokine A20
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-3a protein.
Specificity
This antibody will recognize both native and recombinant human MIP-3a in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Applications
  • Western blot (1:500 - 1:2000)
  • ELISA (1:20000 - 1:100000)
Usage
This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 11 kD in size corresponding to native human MIP-3a protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCL20 / MIP-3-Alpha
P78556 NM_004591 NP_004582.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024