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CCL20 / MIP‑3‑Alpha Antibody LS‑C104608

Catalog Number Size Price
LS-C104608-50 50 µg $285 
LS-C104608-100 100 µg $325 
Western Blot (reducing) of CCL20 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of CCL20 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of CCL20 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of CCL20 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of CCL20 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of CCL20 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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CCL20 / MIP‑3‑Alpha Antibody LS‑C104608

Note: This antibody replaces LS-C54224, LS-C42989, LS-C16275
Antibody:
Rabbit Polyclonal to Human CCL20 / MIP-3-Alpha
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$285 
LS-C104608-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human CCL20 / MIP-3-Alpha
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
MIP-3-Alpha antibody LS-C104608 is an unconjugated rabbit polyclonal antibody to human MIP-3-Alpha (CCL20 ). Validated for ELISA and WB.
Target
Human CCL20 / MIP-3-Alpha
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
E.coli derived recombinant Human MIP-3alpha.
Specificity
Human MIP-3alpha
Usage
Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human colon/rectum adenocarcinoma tissue. The recommended concentration is 0.5ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect Human MIP-3alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIP-3alpha (LS-C104612) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIP-3alpha. Western Blot: To detect HumanMIP-3alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Reconstitute with sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCL20 / MIP-3-Alpha
P78556 NM_004591 NP_004582.1

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Requested From: United States
Date Requested: 9/17/2019