Mouse Ccl12 / MCP5

Rabbit

Mouse (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated. Also available conjugated with Biotin.

Immunoaffinity purified

Unmodified

E.coli derived recombinant Murine MCP-5.

Mouse MCP-5

• Western blot (0.1 - 0.2 µg/ml)
• ELISA (0.5 - 2 µg/ml)

Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of mMCP-5 (1000 ng/ml), a concentration of 2-3 ug/ml of this antibody is required. ELISA: To detect mMCP-5 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine MCP-5 (LS-C104806) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mMCP-5. Western Blot: To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-5 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Lyophilized from PBS, pH 7.2

Reconstitute in sterile water to 0.1-1.0 mg/ml.

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q62401 NCBI: NM_011331 NP_035461.2