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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CCK4 / PTK7 Antibody LS‑C667748
PTK7 antibody LS-C667748 is an unconjugated rabbit polyclonal antibody to human PTK7 (CCK4). Validated for IHC and WB.
Catalog
Size
Price
LS-C667748-50
50 µl (1 mg/ml)
$235
LS-C667748-100
100 µl (1 mg/ml)
$275

Popular CCK4 / PTK7 Products

Anti-PTK7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTK7.
Species: Human
Applications: Immunofluorescence, Western blot, Flow Cytometry
Anti-PTK7 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Immunohistochemistry of PTK7 in human colon tissue with PTK7 antibody at 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-CCK4 / PTK7 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Antibody
Species: Human
Applications: Immunofluorescence, Western blot, ELISA

Product Description

PTK7 antibody LS-C667748 is an unconjugated rabbit polyclonal antibody to human PTK7 (CCK4). Validated for IHC and WB.

Specifications

Target
Human CCK4 / PTK7
Synonyms
PTK7, CCK-4, CCK4, Colon carcinoma kinase 4, Tyrosine-protein kinase-like 7, Protein-tyrosine kinase 7, PTK7 protein tyrosine kinase 7
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity chromatography
Modifications
Unmodified
Applications
  • IHC (1:50 - 1:200)
  • IHC - Paraffin
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CCK4 / PTK7 antibody was raised against recombinant full length protein of human PTK7
Specificity
Recognizes endogenous levels of PTK7 protein.
Usage
Western blot analysis of PTK7 expression in HepG2 (A), Jurkat (B), mouse liver (C), rat testis (D) whole cell lysates. Immunohistochemical analysis of PTK7 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Presentation
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About CCK4 / PTK7
PTK7, a CCK4 membrane spanning tyrosine protein kinase, belongs to a subgroup of kinases that lack detectable catalytic tyrosine kinase activity but retain roles in signal transduction. PTK7 may function as a cell adhesion molecule. This gene was isolated from carcinomic colon tissue and has been suggested to affect colon carcinoma physiology. Expression of PTK7 has been shown to be reduced in melanoma samples. Q13308 NM_002821 NP_002812.2


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Images

Immunohistochemistry - Paraffin

Western blot

Immunohistochemistry - Paraffin

Western blot

Immunohistochemistry - Paraffin

Western blot

Immunohistochemistry - Paraffin

Western blot


Requested From: United States
Date Requested: 11/13/2018

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