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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CCK4 / PTK7 Antibody IHC‑plus™ LS‑B6561
Note: This antibody replaces LS-C47365
PTK7 antibody LS-B6561 is an unconjugated rabbit polyclonal antibody to PTK7 (CCK4) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B6561-50
50 µl
$425
Description
PTK7 antibody LS-B6561 is an unconjugated rabbit polyclonal antibody to PTK7 (CCK4) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CCK4 / PTK7
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Horse, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • Western blot (1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Immunogen
CCK4 / PTK7 antibody was raised against a portion of amino acid 900-950 of human PTK7 protein.
Specificity
A portion of amino acid 900-950 of human PTK7 protein.
Presentation
Crude rabbit sera with 50% glycerol and 0.025% sodium azide.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
About CCK4 / PTK7
Q13308 NM_002821 NP_002812.2

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Images

Immunohistochemistry

Anti-PTK7 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-PTK7 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Western blot

Western blot of PTK7 in HCT116 cell lysate. Lane 1 shows pre-immune sera. Lane 2 shows antibody tested at 1:5000 dilution.
Western blot of PTK7 in HCT116 cell lysate. Lane 1 shows pre-immune sera. Lane 2 shows antibody tested at 1:5000 dilution.

Immunohistochemistry

Anti-PTK7 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-PTK7 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Western blot

Western blot of PTK7 in HCT116 cell lysate. Lane 1 shows pre-immune sera. Lane 2 shows antibody tested at 1:5000 dilution.
Western blot of PTK7 in HCT116 cell lysate. Lane 1 shows pre-immune sera. Lane 2 shows antibody tested at 1:5000 dilution.

Immunohistochemistry

Anti-PTK7 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-PTK7 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Western blot

Western blot of PTK7 in HCT116 cell lysate. Lane 1 shows pre-immune sera. Lane 2 shows antibody tested at 1:5000 dilution.
Western blot of PTK7 in HCT116 cell lysate. Lane 1 shows pre-immune sera. Lane 2 shows antibody tested at 1:5000 dilution.

Immunohistochemistry

Anti-PTK7 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-PTK7 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Western blot

Western blot of PTK7 in HCT116 cell lysate. Lane 1 shows pre-immune sera. Lane 2 shows antibody tested at 1:5000 dilution.
Western blot of PTK7 in HCT116 cell lysate. Lane 1 shows pre-immune sera. Lane 2 shows antibody tested at 1:5000 dilution.

Requested From: United States
Date Requested: 11/18/2018
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