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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CBX5 / HP1 Alpha Antibody (aa1‑100) LS‑C2692
HP1 Alpha antibody LS-C2692 is an unconjugated rabbit polyclonal antibody to human HP1 Alpha (CBX5). Validated for WB.
Catalog
Size
Price
LS-C2692-100
100 µl
$335

Popular CBX5 / HP1 Alpha Products

Immunohistochemistry of paraffin-embedded human mammary cancer using CBX5 antibodyat dilution of 1:100 (40x lens).
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Anti-CBX5 / HP1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2394 concentration 5 ug/ml.
Species: Human, Monkey, Bovine, Horse, Pig, Sheep
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemical analysis of HP1 alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Bovine
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
Immunohistochemistry of paraffin-embedded human stomach cancer using CBX5 antibody at dilution of 1:200 (400x lens).
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation, ChIP-sequencing
Antibody
Species: Mouse, Human
Applications: IHC, ICC, Western blot, ELISA

Product Description

HP1 Alpha antibody LS-C2692 is an unconjugated rabbit polyclonal antibody to human HP1 Alpha (CBX5). Validated for WB.

Specifications

Target
Human CBX5 / HP1 Alpha
Synonyms
CBX5, Chromobox homolog 5, HP1Hs alpha, HP1Hs-alpha, HP1, HP1 alpha homolog, HP1 alpha, HP1-ALPHA, HP1A, Antigen p25, Chromobox protein homolog 5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Purification
Purified IgG
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:1000)
  • (applications tested for the base form of this product only)
Immunogen
A synthetic peptide containing an N-terminal region of human HP 1 alpha (within residues 1-100). [Swiss-Prot P45973]
Epitope
aa1-100
Specificity
HP 1 alpha.
Presentation
PBS, 0.02% Sodium Azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About CBX5 / HP1 Alpha
CBX5 / HP1 Alpha is a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. P45973 NM_012117 NP_036249.1


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Images

Western blot

Detection of HP 1 alpha in HeLa whole cell lysate using LS-C2692. 1 min. ECL exposure.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of HP 1 alpha in HeLa whole cell lysate using LS-C2692. 1 min. ECL exposure. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of HP 1 alpha in HeLa whole cell lysate using LS-C2692. 1 min. ECL exposure.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of HP 1 alpha in HeLa whole cell lysate using LS-C2692. 1 min. ECL exposure. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of HP 1 alpha in HeLa whole cell lysate using LS-C2692. 1 min. ECL exposure.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of HP 1 alpha in HeLa whole cell lysate using LS-C2692. 1 min. ECL exposure. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of HP 1 alpha in HeLa whole cell lysate using LS-C2692. 1 min. ECL exposure.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of HP 1 alpha in HeLa whole cell lysate using LS-C2692. 1 min. ECL exposure. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 10/17/2018

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