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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CBFB Antibody LS‑C394113
CBFB antibody LS-C394113 is an unconjugated rabbit polyclonal antibody to human CBFB. Validated for WB.
Catalog
Size
Price
LS-C394113-100
100 µl
$305

Popular CBFB Products

Formalin-fixed and paraffin-embedded human brain tissue reacted with CBFB Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Immunohistochemical analysis of CBFB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Pig, Zebrafish
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Anti-CBFB antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7059 dilution 1:100.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CBFB mAb.
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Dot Blot
Western blot of PEBP2beta antibody
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

CBFB antibody LS-C394113 is an unconjugated rabbit polyclonal antibody to human CBFB. Validated for WB.
About CBFB
CBFB is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). Q13951 NM_001755 NP_001746.1

CBFB Antibody, CBF-beta Antibody, PEBP2-beta Antibody, PEA2-beta Antibody, PEBP2B Antibody


Specifications

Target
Human CBFB
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • Western blot
Immunogen
CBFB antibody was raised against synthetic peptide.
Specificity
Human CBFB
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Western blot

All lanes: Anti-CBFB Antibody at 1:1000 dilution. Lane 1: Raji whole cell lysate. Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CBFB Antibody at 1:1000 dilution. Lane 1: Raji whole cell lysate. Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot

All lanes: Anti-CBFB Antibody at 1:1000 dilution. Lane 1: Raji whole cell lysate. Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CBFB Antibody at 1:1000 dilution. Lane 1: Raji whole cell lysate. Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot

All lanes: Anti-CBFB Antibody at 1:1000 dilution. Lane 1: Raji whole cell lysate. Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CBFB Antibody at 1:1000 dilution. Lane 1: Raji whole cell lysate. Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot

All lanes: Anti-CBFB Antibody at 1:1000 dilution. Lane 1: Raji whole cell lysate. Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CBFB Antibody at 1:1000 dilution. Lane 1: Raji whole cell lysate. Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Requested From: United States
Date Requested: 8/20/2018

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