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CASZ1 Antibody (Internal) LS‑C154253

Catalog Number Size Price
LS-C154253-100 100 µg (1.07 mg/ml) $425 
Anti-hCASZ1 Antibody - Western Blot. Western blot of anti-hCASZ1 antibody. This blot shows detection of endogenous and transfected human CASZ1 protein in fresh whole cell lysate (~30 ug). Protein was resolved by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1000, incubated 1.5 hours at room temperature, and incubated with HRP-conjugated Goat Anti-Rabbit antibody for 45 min. at room temperature. Lane 1, BE2(s) cell lysate. Lane 2, BE2(N) cell lysate. Lane 3, SY5Y transfected with hCASZ5 (125kD). Lane 4, SY5Y transfected with hCASZ11 (190kD). Personal communication, Carol Thiele, NCI, Bethesda, MD.

CASZ1 Antibody (Internal) LS‑C154253

Antibody:
Rabbit Polyclonal to Human CASZ1
Application:
WB, ELISA
Reactivity:
Human, Mouse, Drosophila, Macaque
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C154253-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human CASZ1
Application:
WB, ELISA
Reactivity:
Human, Mouse, Drosophila, Macaque
Format:
Unconjugated, Unmodified

Specifications

Description
CASZ1 antibody LS-C154253 is an unconjugated rabbit polyclonal antibody to CASZ1 from human, mouse, drosophila and other species. Validated for ELISA and WB.
Target
Human CASZ1
Host
Rabbit
Reactivity
Human, Mouse, Drosophila, Macaque (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:1000 - 1:5000)
  • ELISA
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human Casz1 protein.
Epitope
Internal
Specificity
A BLAST analysis was used to suggest reactivity with CASZ1 proteins from human, mouse, Drosophila, chimpanzee, and macaque based on a 100% homology. Partial reactivity is expected with horse and dog CASZ1 based on a 92% homology with the immunizing sequence. Cross-reactivity with CASZ1 from other sources has not been determined.
Usage
This antibody has been tested for use in ELISA and western blotting. This antiserum detects endogenous CASZ1 proteins, both the hCASZ5 and hCASZ11 isoforms. Expect a band approximately 125 kD for hCASZ5 and 190 kD for hCASZ11 in size corresponding CASZ1 by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Presentation
Sterile Solution 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CASZ1
Q86V15 NM_017766 NP_060236.3

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Requested From: United States
Date Requested: 8/20/2019
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