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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-C154253-100 100 µg (1.07 mg/ml) $425 
CASZ1 Antibody - Anti-hCASZ1 Antibody - Western Blot. Western blot of anti-hCASZ1 antibody. This blot shows detection of endogenous and transfected human CASZ1 protein in fresh whole cell lysate (~30 ug). Protein was resolved by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1000, incubated 1.5 hours at room temperature, and incubated with HRP-conjugated Goat Anti-Rabbit antibody for 45 min. at room temperature. Lane 1, BE2(s) cell lysate. Lane 2, BE2(N) cell lysate. Lane 3, SY5Y transfected with hCASZ5 (125kD). Lane 4, SY5Y transfected with hCASZ11 (190kD). Personal communication, Carol Thiele, NCI, Bethesda, MD.

Polyclonal Rabbit anti‑Human CASZ1 Antibody (IHC, IF, WB) LS‑C154253

Polyclonal Rabbit anti‑Human CASZ1 Antibody (IHC, IF, WB) LS‑C154253

Antibody:
Rabbit Polyclonal to Human CASZ1
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Chimpanzee, Mouse, Drosophila, Macaque
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C154253-100
100 µg
1.07 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human CASZ1
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Chimpanzee, Mouse, Drosophila, Macaque
Format:
Unconjugated, Unmodified

Specifications

Description
CASZ1 antibody LS-C154253 is an unconjugated rabbit polyclonal antibody to CASZ1 from human. It is reactive with human, mouse, chimpanzee and other species. Validated for ELISA, IF, IHC and WB.
Target
Human CASZ1
Synonyms
CASZ1 | Castor-related protein | CAS11 | Castor | Castor homolog 1, zinc finger | CST | DJ734G22.1 | Zinc finger protein 693 | SRG | ZNF693 | Castor zinc finger 1 | Survival-related
Host
Rabbit
Reactivity
Human, Chimpanzee, Mouse, Drosophila, Macaque (tested or 100% immunogen sequence identity)
Predicted
Human, Chimpanzee, Mouse, Drosophila, Macaque (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • Immunofluorescence
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:45000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human Casz1 protein.
Specificity
A BLAST analysis was used to suggest reactivity with CASZ1 proteins from human, mouse, Drosophila, chimpanzee, and macaque based on a 100% homology. Partial reactivity is expected with horse and dog CASZ1 based on a 92% homology with the immunizing sequence. Cross-reactivity with CASZ1 from other sources has not been determined.
Usage
This antibody has been tested for use in ELISA and western blotting. This antiserum detects endogenous CASZ1 proteins, both the hCASZ5 and hCASZ11 isoforms. Expect a band approximately 125 kD for hCASZ5 and 190 kD for hCASZ11 in size corresponding CASZ1 by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CASZ1
Q86V15 NM_017766 NP_060236.3

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Requested From: United States
Date Requested: 6/6/2020