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Catalog Number Size Price
LS-C745339-25 25 µl (1 mg/ml) $304 
CASZ1 Antibody - Immunohistochemistry results of rabbit Anti-hCASZ1 Antibody. Tissue: NB patient tumor. A. Score 0- a rare positive nuclei. B. Score 1- (1-10% positive) equivocal/uninterpretable. C. Score 2- (10-50% positive) weak positive. D. Score 3- (>50% positive) strong positive. Primary Antibody: Rabbit Anti-CASZ1 stained brown. Nucleus counterstained with hematoxylin (blue). Localization: Nuclear.
CASZ1 Antibody - Immunohistochemistry results of rabbit Anti-hCasz1 Antibody. Tissue: NB patient tumor. A. CASZ1 localized exclusively in the cytoplasm. B. CASZ1 localized in the cytoplasm and nucleus. Primary Antibody: Rabbit Anti-CASZ1 stained brown. Nucleus counterstained with hematoxylin (blue).
CASZ1 Antibody - Immunofluorescence of rabbit anti-CASZ1 Antibody. Tissue: adult murine ocular tissue. Antibody: Rabbit Anti-CASZ1 Antibody. Counterstain: DAPI. Localization: nucleus in lens epithelia but primarily localizes in the cytoplasm in photoreceptor cells.
CASZ1 Antibody - Immunofluorescence results of rabbit Anti-CASZ1 Antibody. Tissue: Mouse Xenograft tumor of human NB cell line transfected with or without tetracycline inducible CASZ1 (NGPtetCASZ1a). Antibody: Rabbit Anti-CASZ1 Antibody. Counterstain: DAPI.
CASZ1 Antibody - Immunofluorescence results of Endogenous CASZ1. Cells: BE2 cells. Pre-Incubation of Anti-CASZ1 Antibody with CASZ1 Peptide: Bottom. Staining: Rabbit Anti-CASZ1 Antibody. Chromatin counter stain: DAPI.
CASZ1 Antibody - Western blot using the anti-hCASZ1 antibody. This blot shows detection of endogenous and transfected human CASZ1 protein in fresh whole cell lysate (~30 µg). Protein was resolved by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000, incubated 1.5 hours at room temperature, and incubated with HRP-conjugated Goat Anti-Rabbit antibody for 45 min. at room temperature. Lane 1, BE2(s) cell lysate. Lane 2, BE2(N) cell lysate. Lane 3, SY5Y transfected with hCas5 (125kDa). Lane 4, SY5Y transfected with hCas11 (190kDa).
CASZ1 Antibody - Western Blot of Anti-CASZ1 Antibody. Lane 1: NBLS Cytoplasmic (20µg). Lane 2: NBLS Nuclear (3µg). Lane 3: BE2C Cytoplasmic (30µg). Lane 4: BE2C Nuclear (7µg). Lane 5: SY5Y-CASZ1b (10µg). Block: 5% Blotto/TTBS for 1 hour. Primary: Casz1 1:10,000 for 1 hour. Secondary: Goat anti-Rabbit HRP for 1 hour. 240sec exposure. Detects nuclear endogenous CASZ1a and CASZ1b; and transiently transfected CASZ1b isoform.
CASZ1 Antibody - Immunohistochemistry results of rabbit Anti-hCASZ1 Antibody. Tissue: NB patient tumor. A. Score 0- a rare positive nuclei. B. Score 1- (1-10% positive) equivocal/uninterpretable. C. Score 2- (10-50% positive) weak positive. D. Score 3- (>50% positive) strong positive. Primary Antibody: Rabbit Anti-CASZ1 stained brown. Nucleus counterstained with hematoxylin (blue). Localization: Nuclear.
CASZ1 Antibody - Immunohistochemistry results of rabbit Anti-hCasz1 Antibody. Tissue: NB patient tumor. A. CASZ1 localized exclusively in the cytoplasm. B. CASZ1 localized in the cytoplasm and nucleus. Primary Antibody: Rabbit Anti-CASZ1 stained brown. Nucleus counterstained with hematoxylin (blue).
CASZ1 Antibody - Immunofluorescence of rabbit anti-CASZ1 Antibody. Tissue: adult murine ocular tissue. Antibody: Rabbit Anti-CASZ1 Antibody. Counterstain: DAPI. Localization: nucleus in lens epithelia but primarily localizes in the cytoplasm in photoreceptor cells.
CASZ1 Antibody - Immunofluorescence results of rabbit Anti-CASZ1 Antibody. Tissue: Mouse Xenograft tumor of human NB cell line transfected with or without tetracycline inducible CASZ1 (NGPtetCASZ1a). Antibody: Rabbit Anti-CASZ1 Antibody. Counterstain: DAPI.
CASZ1 Antibody - Immunofluorescence results of Endogenous CASZ1. Cells: BE2 cells. Pre-Incubation of Anti-CASZ1 Antibody with CASZ1 Peptide: Bottom. Staining: Rabbit Anti-CASZ1 Antibody. Chromatin counter stain: DAPI.
CASZ1 Antibody - Western blot using the anti-hCASZ1 antibody. This blot shows detection of endogenous and transfected human CASZ1 protein in fresh whole cell lysate (~30 µg). Protein was resolved by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000, incubated 1.5 hours at room temperature, and incubated with HRP-conjugated Goat Anti-Rabbit antibody for 45 min. at room temperature. Lane 1, BE2(s) cell lysate. Lane 2, BE2(N) cell lysate. Lane 3, SY5Y transfected with hCas5 (125kDa). Lane 4, SY5Y transfected with hCas11 (190kDa).
CASZ1 Antibody - Western Blot of Anti-CASZ1 Antibody. Lane 1: NBLS Cytoplasmic (20µg). Lane 2: NBLS Nuclear (3µg). Lane 3: BE2C Cytoplasmic (30µg). Lane 4: BE2C Nuclear (7µg). Lane 5: SY5Y-CASZ1b (10µg). Block: 5% Blotto/TTBS for 1 hour. Primary: Casz1 1:10,000 for 1 hour. Secondary: Goat anti-Rabbit HRP for 1 hour. 240sec exposure. Detects nuclear endogenous CASZ1a and CASZ1b; and transiently transfected CASZ1b isoform.
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Polyclonal Rabbit anti‑Human CASZ1 Antibody (IHC, IF, WB) LS‑C745339

Polyclonal Rabbit anti‑Human CASZ1 Antibody (IHC, IF, WB) LS‑C745339

Antibody:
CASZ1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Chimpanzee, Mouse, Drosophila, Macaque
Format:
Unconjugated, Unmodified
Price
Catalog Number
$304
LS-C745339-25
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CASZ1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Chimpanzee, Mouse, Drosophila, Macaque
Format:
Unconjugated, Unmodified

Specifications

Description
CASZ1 antibody LS-C745339 is an unconjugated rabbit polyclonal antibody to CASZ1 from human. It is reactive with human, mouse, chimpanzee and other species. Validated for ELISA, IF, IHC and WB.
Target
Human CASZ1
Synonyms
CASZ1 | Castor-related protein | CAS11 | Castor | Castor homolog 1, zinc finger | CST | DJ734G22.1 | Zinc finger protein 693 | SRG | ZNF693 | Castor zinc finger 1 | Survival-related
Host
Rabbit
Reactivity
Human, Chimpanzee, Mouse, Drosophila, Macaque (tested or 100% immunogen sequence identity)
Predicted
Human, Chimpanzee, Mouse, Drosophila, Macaque (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human Casz1 protein.
Specificity
A BLAST analysis was used to suggest reactivity with CASZ1 proteins from human, mouse, Drosophila, chimpanzee, and macaque based on a 100% homology. Partial reactivity is expected with horse and dog CASZ1 based on a 92% homology with the immunizing sequence. Cross-reactivity with CASZ1 from other sources has not been determined.
Applications
  • IHC
  • Immunofluorescence
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:45000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CASZ1
Q86V15 NM_017766 NP_060236.3

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/19/2024