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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CASR/Calcium Sensing Receptor Antibody (N‑Terminus, clone HL 1499, DY488) LS‑C140886
Calcium Sensing Receptor antibody LS-C140886 is a DY488-conjugated mouse monoclonal antibody to Calcium Sensing Receptor (CASR) from human, mouse, rat and other species. Validated for Flow, ICC, IHC, IP, RIA and WB.
Catalog
Size
Price
LS-C140886-100
100 µl (0.7 mg/ml)
$365
Description
Calcium Sensing Receptor antibody LS-C140886 is a DY488-conjugated mouse monoclonal antibody to Calcium Sensing Receptor (CASR) from human, mouse, rat and other species. Validated for Flow, ICC, IHC, IP, RIA and WB.
Target
Human CASR/Calcium Sensing Receptor
Host
Mouse
Reactivity
Human, Mouse, Rat, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [HL 1499]
Conjugations
DyLight 488. Also available Unconjugated or conjugated with Biotin, DY650, DY550.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • ICC
  • Western blot (1:50 - 1:200)
  • Immunoprecipitation
  • Flow Cytometry
  • Radioimmunoassay
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
N-terminal, extracellular region of human Calcium Sensing Receptor (see reference 2)
Epitope
N-Terminus
Specificity
Calcium Sensing Receptor protein.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM Sodium Borate, 0.05% Sodium Azide
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
About CASR/Calcium Sensing Receptor
P41180 NM_000388 NP_000379.2

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Images

Immunohistochemistry

Anti-Calcium Sensing Receptor antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Calcium Sensing Receptor antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Calcium Sensing Receptor Antibody (HL 1499) - Western blot analysis of Calcium Sensing Receptor expression in rat kidney tissue.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Calcium Sensing Receptor Antibody (HL 1499) - Western blot analysis of Calcium Sensing Receptor expression in rat kidney tissue. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of Calcium Sensing Receptor in transfected 293 cell lysate. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of Calcium Sensing Receptor in transfected 293 cell lysate. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-Calcium Sensing Receptor antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Calcium Sensing Receptor antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Calcium Sensing Receptor Antibody (HL 1499) - Western blot analysis of Calcium Sensing Receptor expression in rat kidney tissue.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Calcium Sensing Receptor Antibody (HL 1499) - Western blot analysis of Calcium Sensing Receptor expression in rat kidney tissue. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of Calcium Sensing Receptor in transfected 293 cell lysate. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of Calcium Sensing Receptor in transfected 293 cell lysate. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-Calcium Sensing Receptor antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Calcium Sensing Receptor antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Calcium Sensing Receptor Antibody (HL 1499) - Western blot analysis of Calcium Sensing Receptor expression in rat kidney tissue.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Calcium Sensing Receptor Antibody (HL 1499) - Western blot analysis of Calcium Sensing Receptor expression in rat kidney tissue. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of Calcium Sensing Receptor in transfected 293 cell lysate. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of Calcium Sensing Receptor in transfected 293 cell lysate. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-Calcium Sensing Receptor antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Calcium Sensing Receptor antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Calcium Sensing Receptor Antibody (HL 1499) - Western blot analysis of Calcium Sensing Receptor expression in rat kidney tissue.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Calcium Sensing Receptor Antibody (HL 1499) - Western blot analysis of Calcium Sensing Receptor expression in rat kidney tissue. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of Calcium Sensing Receptor in transfected 293 cell lysate. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of Calcium Sensing Receptor in transfected 293 cell lysate. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/17/2018
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