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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CASQ1 / Calsequestrin 1 Antibody LS‑C155368
Calsequestrin 1 antibody LS-C155368 is an unconjugated rabbit polyclonal antibody to Calsequestrin 1 (CASQ1) from human, mouse and rat. Validated for WB.
Catalog
Size
Price
LS-C155368-100
100 µl (0.79 mg/ml)
$335

Popular CASQ1 / Calsequestrin 1 Products

Immunohistochemistry of paraffin-embedded human gastric cancer using antibody at dilution of 1:100.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, IHC - Paraffin, ELISA
Antibody
Species: Rabbit
Applications: Immunofluorescence, Western blot

Product Description

Calsequestrin 1 antibody LS-C155368 is an unconjugated rabbit polyclonal antibody to Calsequestrin 1 (CASQ1) from human, mouse and rat. Validated for WB.

Specifications

Target
Human CASQ1 / Calsequestrin 1
Synonyms
CASQ1, CASQ, Calsequestrin-1, Calmitine, PDIB1, Calmitin
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:3000)
Immunogen
Recombinant fragment corresponding to a region within amino acids 136 and 381 of Calsequestrin 1 (SwissProt P31415). Percent identity by BLAST analysis:
Specificity
Human, mouse, rat CASQ1
Usage
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Presentation
PBS, pH 7.0, 0.01% Thimerosal, 20% Glycerol, 1% BSA
Storage
Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About CASQ1 / Calsequestrin 1
Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. Calcium ions are bound by clusters of acidic residues at the protein surface, often at the interface between subunits. Can bind around 80 Ca2+ ions. Regulates the release of lumenal Ca2+ via the calcium release channel RYR1; this plays an important role in triggering muscle contraction. P31415 NM_001231 NP_001222.3


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Images

Western blot

Sample (50 ug of whole cell lysate) A: Rat heart 10% SDS PAGE CASQ1 antibody diluted at 1:1000
Sample (50 ug of whole cell lysate) A: Rat heart 10% SDS PAGE CASQ1 antibody diluted at 1:1000

Western blot

Sample (50 ug of whole cell lysate) A: mouse heart 10% SDS PAGE CASQ1 antibody diluted at 1:1000
Sample (50 ug of whole cell lysate) A: mouse heart 10% SDS PAGE CASQ1 antibody diluted at 1:1000

Western blot

Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE CASQ1 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE CASQ1 antibody diluted at 1:1000

Western blot

Sample (50 ug of whole cell lysate) A: Rat heart 10% SDS PAGE CASQ1 antibody diluted at 1:1000
Sample (50 ug of whole cell lysate) A: Rat heart 10% SDS PAGE CASQ1 antibody diluted at 1:1000

Western blot

Sample (50 ug of whole cell lysate) A: mouse heart 10% SDS PAGE CASQ1 antibody diluted at 1:1000
Sample (50 ug of whole cell lysate) A: mouse heart 10% SDS PAGE CASQ1 antibody diluted at 1:1000

Western blot

Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE CASQ1 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE CASQ1 antibody diluted at 1:1000

Western blot

Sample (50 ug of whole cell lysate) A: Rat heart 10% SDS PAGE CASQ1 antibody diluted at 1:1000
Sample (50 ug of whole cell lysate) A: Rat heart 10% SDS PAGE CASQ1 antibody diluted at 1:1000

Western blot

Sample (50 ug of whole cell lysate) A: mouse heart 10% SDS PAGE CASQ1 antibody diluted at 1:1000
Sample (50 ug of whole cell lysate) A: mouse heart 10% SDS PAGE CASQ1 antibody diluted at 1:1000

Western blot

Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE CASQ1 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE CASQ1 antibody diluted at 1:1000

Western blot

Sample (50 ug of whole cell lysate) A: Rat heart 10% SDS PAGE CASQ1 antibody diluted at 1:1000
Sample (50 ug of whole cell lysate) A: Rat heart 10% SDS PAGE CASQ1 antibody diluted at 1:1000

Western blot

Sample (50 ug of whole cell lysate) A: mouse heart 10% SDS PAGE CASQ1 antibody diluted at 1:1000
Sample (50 ug of whole cell lysate) A: mouse heart 10% SDS PAGE CASQ1 antibody diluted at 1:1000

Western blot

Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE CASQ1 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE CASQ1 antibody diluted at 1:1000

Requested From: United States
Date Requested: 11/13/2018

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