Caspase 9 antibody LS-C12593 is an unconjugated mouse monoclonal antibody to human Caspase 9 (CASP9). Validated for ELISA and WB.
Human CASP9 / Caspase 9
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- Western blot
- (applications tested for the base form of this product only)
The N-terminal fragment of human Caspase 9 (amino acid residues 1-134).
Recognizes the proform and the active cleaved form of Caspase 9, Mr 46kD and 34kD, respectively. Does not recognize Caspase 3, 6, 7 and 8. Species cross-reactivity: Human, not mouse or rat.
Suitable for use in ELISA and Western Blot. Western Blot Analysis: 0.5-2 ug/ml detects the proform of Caspase 9 in RIPA lysates from both A431 and Raji cells. 0.5-2 ug/ml of a previous lot detected the proform and cleaved Caspase 9 in RIPA lysates from HFF cells. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Caspase 9 (2 ug/ml). Proteins were visualized using a goat antimouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.