Human CASP9 / Caspase 9
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (1:1000 - 1:5000)
- IHC - Frozen
- Western blot (1:1000 - 1:2000)
- Immunoprecipitation (1:50 - 1:200)
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including antigen retrieval solutions, blocking agents
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A synthetic peptide corresponding to amino acids 112-130 (RPEIRKPEVLRPETPRPVD) of human caspase-9, GenBank no. gi|55960247|emb|CAI12974.1|. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey (100%); Gibbon (95%); Mouse (74%); Rat (63%).
This polyclonal antisera recognizes the proform of caspase-9 (~50 kD), and the large (~35 kD) subunit (with pro-domain sequences) of active/cleaved Caspase-9. It does not recognize the small (~15 kD) subunit of active/cleaved Caspase-9. Intermediate caspase-9 cleavage forms may also be seen at ~21 kD.
Antiserum, 0.05% sodium azide
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.