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Caspase 8 antibody LS-C507 is an unconjugated mouse monoclonal antibody to Caspase 8 (CASP8) from human, mouse and monkey. Validated for Flow and WB.
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Review This ProductPopular CASP8 / Caspase 8 Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat
Applications:
Western blot, Immunoprecipitation
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Product Description
Caspase 8 antibody LS-C507 is an unconjugated mouse monoclonal antibody to Caspase 8 (CASP8) from human, mouse and monkey. Validated for Flow and WB.
Specifications
Target
Human CASP8 / Caspase 8
Synonyms
CASP8, ALPS2B, Apoptotic protease Mch-5, Apoptotic cysteine protease, Casp-8, Caspase-8, CAP4, Caspase 8, MACH, MCH5, MORT1-associated ced-3 homolog, ICE-like apoptotic protease 5, Pro Caspase 8, FADD-like ICE, FLICE, MACH-alpha-1/2/3 protein, MACH-beta-1/2/3/4 protein
Host
Mouse
Reactivity
Human, Monkey, Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
[90A992]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
- Western blot (0.5 - 2 µg/ml)
- Flow Cytometry
Failed Applications
- IHC - Paraffin
Immunogen
Amino acids PVETDSEEQPof human Caspase-8 were used as the immunogen for this antibody. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Mosquito, Nematode, Arabidopsis, Coccidioides (80%).
Epitope
aa217-222
Specificity
This can be used for detection of the pro-form of Caspase-8.
Presentation
PBS, 0.02% Sodium Azide
Storage
Short term: 4°C; Long term: -70°C.
Restrictions
For research use only.
About CASP8 / Caspase 8
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases.
Q14790
nm_001228
AAC50645.1
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Images
Western blot
Western blot analysis for human Caspase-8 using Jurkat lysates with antibody at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2). antibody only detects 55 kD Caspase-8 in Jurkat cells.
Flow Cytometry
Flow cytometry of Caspase-8 in HeLa cells using 0.1 ug of Caspase-8 antibody. Shaded histogram represents cells without antibody; green represents isotype control, ; red represents Caspase-8 antibody. Goat anti-mouse IgG-FITC secondary antibody, was used for this test.
Western blot
Western blot analysis for human Caspase-8 using Jurkat lysates with antibody at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2). antibody only detects 55 kD Caspase-8 in Jurkat cells.
Flow Cytometry
Flow cytometry of Caspase-8 in HeLa cells using 0.1 ug of Caspase-8 antibody. Shaded histogram represents cells without antibody; green represents isotype control, ; red represents Caspase-8 antibody. Goat anti-mouse IgG-FITC secondary antibody, was used for this test.
Western blot
Western blot analysis for human Caspase-8 using Jurkat lysates with antibody at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2). antibody only detects 55 kD Caspase-8 in Jurkat cells.
Flow Cytometry
Flow cytometry of Caspase-8 in HeLa cells using 0.1 ug of Caspase-8 antibody. Shaded histogram represents cells without antibody; green represents isotype control, ; red represents Caspase-8 antibody. Goat anti-mouse IgG-FITC secondary antibody, was used for this test.
Western blot
Western blot analysis for human Caspase-8 using Jurkat lysates with antibody at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2). antibody only detects 55 kD Caspase-8 in Jurkat cells.
Flow Cytometry
Flow cytometry of Caspase-8 in HeLa cells using 0.1 ug of Caspase-8 antibody. Shaded histogram represents cells without antibody; green represents isotype control, ; red represents Caspase-8 antibody. Goat anti-mouse IgG-FITC secondary antibody, was used for this test.
Requested From: United States
Date Requested: 10/19/2018
Date Requested: 10/19/2018
