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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CASP8 / Caspase 8 Antibody (aa217‑222, clone 90A992) LS‑C507
Caspase 8 antibody LS-C507 is an unconjugated mouse monoclonal antibody to Caspase 8 (CASP8) from human, mouse and monkey. Validated for Flow and WB.
Catalog
Size
Price
LS-C507-100
100 µg (0.5 mg/ml)
$335

Popular CASP8 / Caspase 8 Products

Anti-Caspase 8 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3796 concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: Western blot, Immunoprecipitation
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CASP8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Western blot Image
Species: Human
Applications: Western blot
Western blot analysis of lysates from LOVO cells treated with UV 15', using Caspase 8 (Phospho-Tyr380) Antibody. The lane on the right is blocked with the phospho peptide.
Species: Human
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

Caspase 8 antibody LS-C507 is an unconjugated mouse monoclonal antibody to Caspase 8 (CASP8) from human, mouse and monkey. Validated for Flow and WB.

Specifications

Target
Human CASP8 / Caspase 8
Synonyms
CASP8, ALPS2B, Apoptotic protease Mch-5, Apoptotic cysteine protease, Casp-8, Caspase-8, CAP4, Caspase 8, MACH, MCH5, MORT1-associated ced-3 homolog, ICE-like apoptotic protease 5, Pro Caspase 8, FADD-like ICE, FLICE, MACH-alpha-1/2/3 protein, MACH-beta-1/2/3/4 protein
Host
Mouse
Reactivity
Human, Monkey, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [90A992]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (0.5 - 2 µg/ml)
  • Flow Cytometry
Failed Applications
  • IHC - Paraffin
Immunogen
Amino acids PVETDSEEQPof human Caspase-8 were used as the immunogen for this antibody. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Mosquito, Nematode, Arabidopsis, Coccidioides (80%).
Epitope
aa217-222
Specificity
This can be used for detection of the pro-form of Caspase-8.
Presentation
PBS, 0.02% Sodium Azide
Storage
Short term: 4°C; Long term: -70°C.
Restrictions
For research use only.
About CASP8 / Caspase 8
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Q14790 nm_001228 AAC50645.1


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Images

Western blot

Western blot analysis for human Caspase-8 using Jurkat lysates with antibody at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2). antibody only detects 55 kD Caspase-8 in Jurkat cells.
Western blot analysis for human Caspase-8 using Jurkat lysates with antibody at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2). antibody only detects 55 kD Caspase-8 in Jurkat cells.

Flow Cytometry

Flow cytometry of Caspase-8 in HeLa cells using 0.1 ug of Caspase-8 antibody. Shaded histogram represents cells without antibody; green represents isotype control, ; red represents Caspase-8 antibody. Goat anti-mouse IgG-FITC secondary antibody, was used for this test.
Flow cytometry of Caspase-8 in HeLa cells using 0.1 ug of Caspase-8 antibody. Shaded histogram represents cells without antibody; green represents isotype control, ; red represents Caspase-8 antibody. Goat anti-mouse IgG-FITC secondary antibody, was used for this test.

Western blot

Western blot analysis for human Caspase-8 using Jurkat lysates with antibody at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2). antibody only detects 55 kD Caspase-8 in Jurkat cells.
Western blot analysis for human Caspase-8 using Jurkat lysates with antibody at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2). antibody only detects 55 kD Caspase-8 in Jurkat cells.

Flow Cytometry

Flow cytometry of Caspase-8 in HeLa cells using 0.1 ug of Caspase-8 antibody. Shaded histogram represents cells without antibody; green represents isotype control, ; red represents Caspase-8 antibody. Goat anti-mouse IgG-FITC secondary antibody, was used for this test.
Flow cytometry of Caspase-8 in HeLa cells using 0.1 ug of Caspase-8 antibody. Shaded histogram represents cells without antibody; green represents isotype control, ; red represents Caspase-8 antibody. Goat anti-mouse IgG-FITC secondary antibody, was used for this test.

Western blot

Western blot analysis for human Caspase-8 using Jurkat lysates with antibody at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2). antibody only detects 55 kD Caspase-8 in Jurkat cells.
Western blot analysis for human Caspase-8 using Jurkat lysates with antibody at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2). antibody only detects 55 kD Caspase-8 in Jurkat cells.

Flow Cytometry

Flow cytometry of Caspase-8 in HeLa cells using 0.1 ug of Caspase-8 antibody. Shaded histogram represents cells without antibody; green represents isotype control, ; red represents Caspase-8 antibody. Goat anti-mouse IgG-FITC secondary antibody, was used for this test.
Flow cytometry of Caspase-8 in HeLa cells using 0.1 ug of Caspase-8 antibody. Shaded histogram represents cells without antibody; green represents isotype control, ; red represents Caspase-8 antibody. Goat anti-mouse IgG-FITC secondary antibody, was used for this test.

Western blot

Western blot analysis for human Caspase-8 using Jurkat lysates with antibody at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2). antibody only detects 55 kD Caspase-8 in Jurkat cells.
Western blot analysis for human Caspase-8 using Jurkat lysates with antibody at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2). antibody only detects 55 kD Caspase-8 in Jurkat cells.

Flow Cytometry

Flow cytometry of Caspase-8 in HeLa cells using 0.1 ug of Caspase-8 antibody. Shaded histogram represents cells without antibody; green represents isotype control, ; red represents Caspase-8 antibody. Goat anti-mouse IgG-FITC secondary antibody, was used for this test.
Flow cytometry of Caspase-8 in HeLa cells using 0.1 ug of Caspase-8 antibody. Shaded histogram represents cells without antibody; green represents isotype control, ; red represents Caspase-8 antibody. Goat anti-mouse IgG-FITC secondary antibody, was used for this test.

Requested From: United States
Date Requested: 10/19/2018

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