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Catalog Number Size Price
LS-C343908-10 10 µg $318 
LS-C343908-100 100 µg (0.5 mg/ml) $470 
CASP3 / Caspase 3 Antibody - Caspase-3(P17) antibody IHC-paraffin: Rat Intestine Tissue Lysate.
CASP3 / Caspase 3 Antibody - Western blot analysis of Caspase-3 (P17) using anti-Caspase-3 (P17) antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse brain tissue lysates,Lane 5: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-3 (P17) antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Caspase-3 (P17) at approximately 32KD,19KD. The expected band size for Caspase-3 (P17) is at 32KD,17KD.
CASP3 / Caspase 3 Antibody - Caspase-3(P17) antibody Western blot. Lane 1: Rat Heart Tissue Lysate. Lane 2: Rat Liver Tissue Lysate. Lane 3: Rat Thymus Tissue Lysate. Lane 4: Rat Spleen Tissue Lysate.
CASP3 / Caspase 3 Antibody - Caspase-3(P17) antibody IHC-paraffin: Rat Intestine Tissue Lysate.
CASP3 / Caspase 3 Antibody - Western blot analysis of Caspase-3 (P17) using anti-Caspase-3 (P17) antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse brain tissue lysates,Lane 5: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-3 (P17) antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Caspase-3 (P17) at approximately 32KD,19KD. The expected band size for Caspase-3 (P17) is at 32KD,17KD.
CASP3 / Caspase 3 Antibody - Caspase-3(P17) antibody Western blot. Lane 1: Rat Heart Tissue Lysate. Lane 2: Rat Liver Tissue Lysate. Lane 3: Rat Thymus Tissue Lysate. Lane 4: Rat Spleen Tissue Lysate.
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Polyclonal Rabbit anti‑Mouse CASP3 / Caspase 3 Antibody (aa67‑81, IHC, WB) LS‑C343908

Polyclonal Rabbit anti‑Mouse CASP3 / Caspase 3 Antibody (aa67‑81, IHC, WB) LS‑C343908

Note: This antibody replaces LS-C388117
Antibody:
CASP3 / Caspase 3 Rabbit anti-Mouse Polyclonal (aa67-81) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C343908-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CASP3 / Caspase 3 Rabbit anti-Mouse Polyclonal (aa67-81) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Caspase 3 antibody LS-C343908 is an unconjugated rabbit polyclonal antibody to Caspase 3 (CASP3) (aa67-81) from mouse. It is reactive with mouse and rat. Validated for IHC and WB.
Target
Mouse CASP3 / Caspase 3
Synonyms
CASP3 | Apopain | CASP-3 | Caspase-3 | CPP32B | CPP32 | Cysteine protease CPP32 | SCA-1 | SREBP cleavage activity 1 | Yama | Procaspase3 | Caspase 3 | CPP-32 | PARP cleavage protease | Protein Yama
Host
Rabbit
Reactivity
Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the N-Terminus of mouse Caspase-3(P17)(67-81 aa TDVDAANLRETFMGL), different from the related rat sequence by one amino acid.
Epitope
aa67-81
Specificity
Highest expression in spleen, lung, liver, kidney and heart. Lower expression in brain, skeletal muscle and testis.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CASP3 / Caspase 3
P42574 NM_004346 NP_004337.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/24/2024