Caspase 2 antibody LS-C18764 is an unconjugated rabbit polyclonal antibody to Caspase 2 (CASP2) from mouse and rat. Validated for ELISA and WB.
Rat CASP2 / Caspase 2
(tested or 100% immunogen sequence identity)
- Western blot
- ELISA (1:1500 - 1:6000)
Synthetic peptide from rat CASP2 / Caspase 2.
A the N-terminus of rat Caspase-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH) through a cysteine residue linker.
This affinity purified polyclonal antibody was tested by ELISA and immunoblotting and was found to be reactive with 48 kD mouse and rat caspase-2. A control and rat caspase-2 transfected human 293 cell lysate was used to show by immunoblotting that this antibody detects rat caspase-2. No reaction was observed against human caspase-2. Immunoblot of mouse neuronal cell lysates from wild-type and caspase-2 (-/-) mice (not shown) was used to show that this antibody cross reacts with mouse caspase-2. This product was assayed against; 1.0 ug of immunizing peptide in a standard sandwich ELISA; using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no. LS-C60884
and TMB as a substrate for 30 minutes at room temperature. A 1:750 dilution is recommended for immunoblot.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Store vial at -20°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.
For research use only.