This affinity purified polyclonal antibody was tested by ELISA and immunoblotting and was found to be reactive with 48 kD mouse and rat caspase-2. A control and rat caspase-2 transfected human 293 cell lysate was used to show by immunoblotting that this antibody detects rat caspase-2. No reaction was observed against human caspase-2. Immunoblot of mouse neuronal cell lysates from wild-type and caspase-2 (-/-) mice (not shown) was used to show that this antibody cross reacts with mouse caspase-2. This product was assayed against; 1.0 ug of immunizing peptide in a standard sandwich ELISA; using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no. LS-C60884
and TMB as a substrate for 30 minutes at room temperature. A 1:750 dilution is recommended for immunoblot.