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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CASP2 / Caspase 2 Antibody (N-Terminus) LS-C18764

Caspase 2 antibody LS-C18764 is an unconjugated rabbit polyclonal antibody to Caspase 2 (CASP2) from mouse and rat. Validated for ELISA and WB.
Catalog
Size
Price
LS-C18764-100
100 µg (0.44 mg/ml)
$425
Description
Caspase 2 antibody LS-C18764 is an unconjugated rabbit polyclonal antibody to Caspase 2 (CASP2) from mouse and rat. Validated for ELISA and WB.
Target
Rat CASP2 / Caspase 2
Host
Rabbit
Reactivity
Rat, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • ELISA (1:1500 - 1:6000)
Immunogen
Synthetic peptide from rat CASP2 / Caspase 2.
Epitope
N-Terminus
Specificity
A the N-terminus of rat Caspase-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH) through a cysteine residue linker.
Usage
This affinity purified polyclonal antibody was tested by ELISA and immunoblotting and was found to be reactive with 48 kD mouse and rat caspase-2. A control and rat caspase-2 transfected human 293 cell lysate was used to show by immunoblotting that this antibody detects rat caspase-2. No reaction was observed against human caspase-2. Immunoblot of mouse neuronal cell lysates from wild-type and caspase-2 (-/-) mice (not shown) was used to show that this antibody cross reacts with mouse caspase-2. This product was assayed against; 1.0 ug of immunizing peptide in a standard sandwich ELISA; using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no. LS-C60884 and TMB as a substrate for 30 minutes at room temperature. A 1:750 dilution is recommended for immunoblot.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.
Restrictions
For research use only.
About CASP2 / Caspase 2
P42575 NM_032982 NP_116764.2

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Images

Western blot

Anti-Caspase-2 Antibody - Western Blot. Affinity Purified antibody to Caspase-2 was used at a 1:750 dilution to detect rat caspase-2 in transfected human 292 cell lysates by Western blot. Asterisk indicates a 48 kD caspase-2 protein clearly detected in the rat caspase-2 transfected lysate. No reaction is observed against human caspase-2. Control (lane 1) and transfected (lane 2) lysates were loaded on a 4-20% gel for SDS-PAGE. After primary antibody incubation and washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG (LS-C60865) preceded color development using Amershams substrate system. Other detection methods will yield similar results.
Anti-Caspase-2 Antibody - Western Blot. Affinity Purified antibody to Caspase-2 was used at a 1:750 dilution to detect rat caspase-2 in transfected human 292 cell lysates by Western blot. Asterisk indicates a 48 kD caspase-2 protein clearly detected in the rat caspase-2 transfected lysate. No reaction is observed against human caspase-2. Control (lane 1) and transfected (lane 2) lysates were loaded on a 4-20% gel for SDS-PAGE. After primary antibody incubation and washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG (LS-C60865) preceded color development using Amershams substrate system. Other detection methods will yield similar results.

Western blot

Anti-Caspase-2 Antibody - Western Blot. Affinity Purified antibody to Caspase-2 was used at a 1:750 dilution to detect rat caspase-2 in transfected human 292 cell lysates by Western blot. Asterisk indicates a 48 kD caspase-2 protein clearly detected in the rat caspase-2 transfected lysate. No reaction is observed against human caspase-2. Control (lane 1) and transfected (lane 2) lysates were loaded on a 4-20% gel for SDS-PAGE. After primary antibody incubation and washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG (LS-C60865) preceded color development using Amershams substrate system. Other detection methods will yield similar results.
Anti-Caspase-2 Antibody - Western Blot. Affinity Purified antibody to Caspase-2 was used at a 1:750 dilution to detect rat caspase-2 in transfected human 292 cell lysates by Western blot. Asterisk indicates a 48 kD caspase-2 protein clearly detected in the rat caspase-2 transfected lysate. No reaction is observed against human caspase-2. Control (lane 1) and transfected (lane 2) lysates were loaded on a 4-20% gel for SDS-PAGE. After primary antibody incubation and washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG (LS-C60865) preceded color development using Amershams substrate system. Other detection methods will yield similar results.

Western blot

Anti-Caspase-2 Antibody - Western Blot. Affinity Purified antibody to Caspase-2 was used at a 1:750 dilution to detect rat caspase-2 in transfected human 292 cell lysates by Western blot. Asterisk indicates a 48 kD caspase-2 protein clearly detected in the rat caspase-2 transfected lysate. No reaction is observed against human caspase-2. Control (lane 1) and transfected (lane 2) lysates were loaded on a 4-20% gel for SDS-PAGE. After primary antibody incubation and washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG (LS-C60865) preceded color development using Amershams substrate system. Other detection methods will yield similar results.
Anti-Caspase-2 Antibody - Western Blot. Affinity Purified antibody to Caspase-2 was used at a 1:750 dilution to detect rat caspase-2 in transfected human 292 cell lysates by Western blot. Asterisk indicates a 48 kD caspase-2 protein clearly detected in the rat caspase-2 transfected lysate. No reaction is observed against human caspase-2. Control (lane 1) and transfected (lane 2) lysates were loaded on a 4-20% gel for SDS-PAGE. After primary antibody incubation and washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG (LS-C60865) preceded color development using Amershams substrate system. Other detection methods will yield similar results.

Western blot

Anti-Caspase-2 Antibody - Western Blot. Affinity Purified antibody to Caspase-2 was used at a 1:750 dilution to detect rat caspase-2 in transfected human 292 cell lysates by Western blot. Asterisk indicates a 48 kD caspase-2 protein clearly detected in the rat caspase-2 transfected lysate. No reaction is observed against human caspase-2. Control (lane 1) and transfected (lane 2) lysates were loaded on a 4-20% gel for SDS-PAGE. After primary antibody incubation and washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG (LS-C60865) preceded color development using Amershams substrate system. Other detection methods will yield similar results.
Anti-Caspase-2 Antibody - Western Blot. Affinity Purified antibody to Caspase-2 was used at a 1:750 dilution to detect rat caspase-2 in transfected human 292 cell lysates by Western blot. Asterisk indicates a 48 kD caspase-2 protein clearly detected in the rat caspase-2 transfected lysate. No reaction is observed against human caspase-2. Control (lane 1) and transfected (lane 2) lysates were loaded on a 4-20% gel for SDS-PAGE. After primary antibody incubation and washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG (LS-C60865) preceded color development using Amershams substrate system. Other detection methods will yield similar results.

Requested From: United States
Date Requested: 4/21/2019
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