Caspase 1 antibody LS-B112 is an unconjugated mouse monoclonal antibody to Caspase 1 (CASP1) from human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Human CASP1 / Caspase 1
(tested or 100% immunogen sequence identity)
Rat (at least 90% immunogen sequence identity)
Protein G purified
- IHC - Paraffin (20 µg/ml)
- Western blot (0.5 - 2 µg/ml)
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A synthetic peptide corresponding to amino acids 371-390 RKVRFSFEQPDGRAQMPTTEof human caspase-1.
will recognize full-length Caspase-1 and cleaved caspase-1 forms that retain amino acids 371-390 of the Caspase-1 protein.
Immunohistochemistry: LS-B112 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B112 was determined to be 20 ug/ml.
PBS, 0.05% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.