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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CASP1 / Caspase 1 Antibody IHC‑plus™ LS‑B3200
Note: This antibody replaces LS-C467
Caspase 1 antibody LS-B3200 is an unconjugated rabbit polyclonal antibody to human Caspase 1 (CASP1). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B3200-50
50 µg (1 mg/ml)
$425

Popular CASP1 / Caspase 1 Products

Antibody
Species: Mouse
Applications: Western blot, Flow Cytometry, ELISA
Anti-Caspase 1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Anti-Caspase 1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Species: Human
Applications: IHC, IHC - Paraffin
Antibody
Species: Human, Mouse, Rat
Applications: ICC, Western blot, Immunoprecipitation
Anti-Caspase 1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

Caspase 1 antibody LS-B3200 is an unconjugated rabbit polyclonal antibody to human Caspase 1 (CASP1). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human CASP1 / Caspase 1
Synonyms
CASP1, Caspase-1, CASP-1, CASP1 nirs variant 1, Caspase 1, ICE, ICE-like protease, IL-1 beta-converting enzyme, IL-1BC, IL1BC, IL1BCE, p45, IL1B-convertase, Interleukin-1 beta convertase
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence
  • Western blot (0.5 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E30121 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B3200. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Usage
Immunohistochemistry: LS-B3200 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3200 was determined to be 5 ug/ml. Western Blot: Casp-1 antibody can be used for the detection of Caspase-1 by Western blot at 0.5 ug/ml. Depending on cell lines or tissues used, other cleavage products may be observed.
Presentation
PBS, 0.02% Sodium Azide
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
About CASP1 / Caspase 1
CASP1 / Caspase 1 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. P29466 NM_001223 NP_001214.1


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Images

Immunohistochemistry

Anti-Caspase 1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Caspase 1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-Caspase 1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Caspase 1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of Caspase-1 in HeLa cells with Caspase-1 antibody at 20 ug/ml.
Immunofluorescence of Caspase-1 in HeLa cells with Caspase-1 antibody at 20 ug/ml.

Western blot

Western blot of caspase-1 in HeLa cell lysate with caspase-1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Western blot of caspase-1 in HeLa cell lysate with caspase-1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.

Immunohistochemistry

Anti-Caspase 1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Caspase 1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-Caspase 1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Caspase 1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of Caspase-1 in HeLa cells with Caspase-1 antibody at 20 ug/ml.
Immunofluorescence of Caspase-1 in HeLa cells with Caspase-1 antibody at 20 ug/ml.

Western blot

Western blot of caspase-1 in HeLa cell lysate with caspase-1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Western blot of caspase-1 in HeLa cell lysate with caspase-1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.

Immunohistochemistry

Anti-Caspase 1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Caspase 1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-Caspase 1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Caspase 1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of Caspase-1 in HeLa cells with Caspase-1 antibody at 20 ug/ml.
Immunofluorescence of Caspase-1 in HeLa cells with Caspase-1 antibody at 20 ug/ml.

Western blot

Western blot of caspase-1 in HeLa cell lysate with caspase-1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Western blot of caspase-1 in HeLa cell lysate with caspase-1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.

Immunohistochemistry

Anti-Caspase 1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Caspase 1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-Caspase 1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Caspase 1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of Caspase-1 in HeLa cells with Caspase-1 antibody at 20 ug/ml.
Immunofluorescence of Caspase-1 in HeLa cells with Caspase-1 antibody at 20 ug/ml.

Western blot

Western blot of caspase-1 in HeLa cell lysate with caspase-1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Western blot of caspase-1 in HeLa cell lysate with caspase-1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.

Requested From: United States
Date Requested: 11/13/2018

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