CASP1, Caspase-1, CASP-1, CASP1 nirs variant 1, Caspase 1, ICE, ICE-like protease, IL-1 beta-converting enzyme, IL-1BC, IL1BC, IL1BCE, p45, IL1B-convertase, Interleukin-1 beta convertase
(tested or 100% immunogen sequence identity)
Immunohistochemistry: LS-B3200 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3200 was determined to be 5 ug/ml. Western Blot: Casp-1 antibody can be used for the detection of Caspase-1 by Western blot at 0.5 ug/ml. Depending on cell lines or tissues used, other cleavage products may be observed.
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.