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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CASP1 / Caspase 1 Antibody (C‑Terminus) LS‑C351905
Caspase 1 antibody LS-C351905 is an unconjugated rabbit polyclonal antibody to Caspase 1 (CASP1) from human, mouse and rat. Validated for ICC, IF and WB.
Catalog
Size
Price
LS-C351905-100
100 µl (1 mg/ml)
$245

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Product Description

Caspase 1 antibody LS-C351905 is an unconjugated rabbit polyclonal antibody to Caspase 1 (CASP1) from human, mouse and rat. Validated for ICC, IF and WB.
About CASP1 / Caspase 1
CASP1 / Caspase 1 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. P29466 NM_001223 NP_001214.1

CASP1 Antibody, Caspase-1 Antibody, CASP-1 Antibody, CASP1 nirs variant 1 Antibody, Caspase 1 Antibody, ICE Antibody, ICE-like protease Antibody, IL-1 beta-converting enzyme Antibody, IL-1BC Antibody, IL1BC Antibody, IL1BCE Antibody, p45 Antibody, IL1B-convertase Antibody, Interleukin-1 beta convertase Antibody


Specifications

Target
Human CASP1 / Caspase 1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human Caspase 1.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of Caspase 1 p10 protein.
Presentation
PBS, pH 7.3, 0.01% sodium azide, 30% glycerol.
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunofluorescence

Immunofluorescent analysis of Caspase 1 p10 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunofluorescent analysis of Caspase 1 p10 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot

Western blot analysis of Caspase 1 p10 expression in HeLa (A); mouse spleen (B); rat spleen (C) whole cell lysates.
Western blot analysis of Caspase 1 p10 expression in HeLa (A); mouse spleen (B); rat spleen (C) whole cell lysates.

Immunofluorescence

Immunofluorescent analysis of Caspase 1 p10 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunofluorescent analysis of Caspase 1 p10 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot

Western blot analysis of Caspase 1 p10 expression in HeLa (A); mouse spleen (B); rat spleen (C) whole cell lysates.
Western blot analysis of Caspase 1 p10 expression in HeLa (A); mouse spleen (B); rat spleen (C) whole cell lysates.

Immunofluorescence

Immunofluorescent analysis of Caspase 1 p10 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunofluorescent analysis of Caspase 1 p10 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot

Western blot analysis of Caspase 1 p10 expression in HeLa (A); mouse spleen (B); rat spleen (C) whole cell lysates.
Western blot analysis of Caspase 1 p10 expression in HeLa (A); mouse spleen (B); rat spleen (C) whole cell lysates.

Immunofluorescence

Immunofluorescent analysis of Caspase 1 p10 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunofluorescent analysis of Caspase 1 p10 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot

Western blot analysis of Caspase 1 p10 expression in HeLa (A); mouse spleen (B); rat spleen (C) whole cell lysates.
Western blot analysis of Caspase 1 p10 expression in HeLa (A); mouse spleen (B); rat spleen (C) whole cell lysates.

Requested From: United States
Date Requested: 7/20/2018

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