CASK antibody LS-C4301 is an unconjugated rabbit polyclonal antibody to CASK from human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB.
Human, Mouse, Rat, Dog
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
- IHC - Paraffin
- Immunofluorescence (10 - 15 µg/ml)
- Western blot (0.1 - 1 µg/ml)
- ELISA (0.1 - 1 µg/ml)
- (applications tested for the base form of this product only)
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CASK antibody was raised against recombinant fusion protein derived from amino acids 315-416 of the human CASK/LIN-2 protein 1. This sequence lies approximately 100 residues COOH-terminal to a putative calmodulin-binding domain and is 100% conserved in human, mouse, and rat CASK proteins.
Detects the ~112kD CASK/LIN-2 protein. Species cross-reactivity: human and rat. (Based on sequence homology, reactivity with mouse and canine CASK is likely).
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Immunofluorescence (rat): 10-15 ug/ml. ELISA (immunogen): 0.1-1.0 ug. ml. Western Blot (human and rat): 0.1-1.0 ug/ml. Lysates testing positive in Western Blot include: T84 cells, Caco-2 cells, rat liver lysate and rat kidney. Immunohistochemistry (rat): paraffin.
PBS, pH 7.4, 0.1% Sodium Azide, 40% Glycerol
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.