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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CARS1 / CARS Antibody (aa31‑80) LS‑C30359
CARS antibody LS-C30359 is an unconjugated rabbit polyclonal antibody to human CARS (CARS1). Validated for IHC and WB.
Catalog
Size
Price
LS-C30359-50
50 µg
$365
Description
CARS antibody LS-C30359 is an unconjugated rabbit polyclonal antibody to human CARS (CARS1). Validated for IHC and WB.
Target
Human CARS1 / CARS
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CARS1 / CARS antibody was raised against synthetic peptide located between aa31-80 of human CARS. Percent identity by BLAST analysis: Human (100%).
Blocking Peptide
LS-E14679 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C30359. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa31-80
Specificity
Human CARS
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About CARS1 / CARS
P49589 NM_001751 NP_001742.1

Popular CARS1 / CARS Products

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Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Immunohistochemical analysis of CARS staining in mouse heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA
Immunohistochemistry of paraffin-embedded mouse lung using CARS Antibody at dilution of 1:100 (40x lens).
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
CARS antibody ARP40295_P050-NP_001014438-CARS (cysteinyl-tRNA synthetase) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human lung.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Bovine, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot

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Images

Immunohistochemistry

CARS antibody ARP40297_P050-NP_001014438-CARS (cysteinyl-tRNA synthetase) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human lung.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CARS antibody ARP40297_P050-NP_001014438-CARS (cysteinyl-tRNA synthetase) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human lung. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

CARS antibody ARP40297_P050-NP_001014438-CARS (cysteinyl-tRNA synthetase) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human lung.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CARS antibody ARP40297_P050-NP_001014438-CARS (cysteinyl-tRNA synthetase) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human lung. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

CARS antibody ARP40297_P050-NP_001014438-CARS (cysteinyl-tRNA synthetase) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human lung.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CARS antibody ARP40297_P050-NP_001014438-CARS (cysteinyl-tRNA synthetase) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human lung. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

CARS antibody ARP40297_P050-NP_001014438-CARS (cysteinyl-tRNA synthetase) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human lung.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CARS antibody ARP40297_P050-NP_001014438-CARS (cysteinyl-tRNA synthetase) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human lung. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/13/2018
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