Human CAMK4 / CaMK IV

Rabbit

Human, Chimpanzee, Mouse, Rat, Bovine, Dog (tested or 100% immunogen sequence identity)

Mouse, Rat (at least 90% immunogen sequence identity)

Polyclonal

Unconjugated

Delipidated and defibrinated

Unmodified

This antiserum was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 300-325 of Human CaM Kinase IV protein.

This antiserum is directed against human CaM Kinase IV protein. The product was delipidated, defibrinated followed by buffering and clarification. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, and rat based on 100% homology for the immunogen sequence. Cross reactivity with CaM Kinase IV homologues from other sources has not been determined.

• IHC
• IHC - Paraffin (1:500)
• Western blot (1:500 - 1:2000)
• ELISA (1:5000 - 1:25000)

Immunohistochemistry: LS-B335 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B335 was determined to be 1:500.

Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Reconstitute in 100 µL of deionized water.

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q16566 NCBI: NM_001744 NP_001735.1