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Catalog Number Size Price
LS-C744961-25 25 µl (1.1 mg/ml) $275 
CA2 / Carbonic Anhydrase II Antibody - Immunohistochemistry with anti-carbonic anhydrase II antibody showing carbonic anhydrase II staining in nucleus and cytoplasm of proximal and distal tubules, bowman’s capsule and glomerular podocytes of human kidney (B) and in basal cells of retina of a mouse eye (C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-carbonic anhydrase II antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using either MACH 1 universal HRP polymer detection (human kidney) or MACH 4 universal AP polymer detection system (mouse eye) and visualized with 3’3-diamino-benzidine substrate (DAB) or WARP RED.
CA2 / Carbonic Anhydrase II Antibody - Western Blot of rabbit Anti-Carbonic Anhydrase II primary antibody. Lane 1: Carbonic Anhydrase II. Lane 2: None. Load: 50 ng per lane. Primary antibody: Carbonic Anhydrase II primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase II. Other band(s): None.
CA2 / Carbonic Anhydrase II Antibody - Immunohistochemistry with anti-carbonic anhydrase II antibody showing carbonic anhydrase II staining in nucleus and cytoplasm of proximal and distal tubules, bowman’s capsule and glomerular podocytes of human kidney (B) and in basal cells of retina of a mouse eye (C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-carbonic anhydrase II antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using either MACH 1 universal HRP polymer detection (human kidney) or MACH 4 universal AP polymer detection system (mouse eye) and visualized with 3’3-diamino-benzidine substrate (DAB) or WARP RED.
CA2 / Carbonic Anhydrase II Antibody - Western Blot of rabbit Anti-Carbonic Anhydrase II primary antibody. Lane 1: Carbonic Anhydrase II. Lane 2: None. Load: 50 ng per lane. Primary antibody: Carbonic Anhydrase II primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase II. Other band(s): None.
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Polyclonal Rabbit anti‑Human CA2 / Carbonic Anhydrase II Antibody (Liquid, IHC, WB) LS‑C744961

Polyclonal Rabbit anti‑Human CA2 / Carbonic Anhydrase II Antibody (Liquid, IHC, WB) LS‑C744961

Antibody:
CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal (Liquid) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Liquid
Price
Catalog Number
$275
LS-C744961-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal (Liquid) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Liquid

Specifications

Description
Carbonic Anhydrase II antibody LS-C744961 is an unconjugated rabbit polyclonal antibody to human Carbonic Anhydrase II (CA2). Validated for ELISA, IHC and WB.
Target
Human CA2 / Carbonic Anhydrase II
Synonyms
CA2 | CA-II | CAII | CAC | Car2 | Carbonate dehydratase II | Carbonic anhydrase C | Carbonic anhydrase II | Carbonic dehydratase | Carbonic anhydrase 2 | Carbonic anhydrase B
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Liquid
Immunogen
Carbonic Anhydrase II Antibody was produced by repeated immunizations with human erythrocytes Carbonic Anhydrase II.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Human Erythrocytes]. Cross reactivity against Carbonic Anhydrase II from other tissues and species may occur but have not been specifically determined.
Applications
  • IHC
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:165000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CA2 / Carbonic Anhydrase II
P00918 NM_000067 NP_000058.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/11/2021