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CA2 / Carbonic Anhydrase II

carbonic anhydrase II

Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6.

Gene Name: carbonic anhydrase II
Synonyms: CA2, CA-II, CAII, CAC, Car2, Carbonate dehydratase II, Carbonic anhydrase C, Carbonic anhydrase II, Carbonic dehydratase, Carbonic anhydrase 2, Carbonic anhydrase B
Target Sequences: NM_000067 NP_000058.1 P00918

Publications (6)

1
Carbonic anhydrase II binds to and enhances activity of the Na+/H+ exchanger. Li X, Alvarez B, Casey JR, Reithmeier RA, Fliegel L. The Journal of biological chemistry. 2002 277:36085-91. [PubMed:12138085]
2
Inhibition of cell growth and nuclear factor-kappaB activity in pancreatic cancer cell lines by a tylophorine analogue, DCB-3503. Shiah HS, Gao W, Baker DC, Cheng YC. Molecular cancer therapeutics. 2006 5:2484-93. [PubMed:17041092]
3
Catalysis and pH control by membrane-associated carbonic anhydrase IX in MDA-MB-231 breast cancer cells. Li Y, Tu C, Wang H, Silverman DN, Frost SC. The Journal of biological chemistry. 2011 286:15789-96. [PubMed:21454639] [PMC:PMC3091188]
4
Role of zinc in catalytic activity of carbonic anhydrase IX. Tu C, Foster L, Alvarado A, McKenna R, Silverman DN, Frost SC. Archives of biochemistry and biophysics. 2012 521:90-4. (WB; Human) [PubMed:22465027] [PMC:PMC3345035]
5
Loss of carbonic anhydrase XII function in individuals with elevated sweat chloride concentration and pulmonary airway disease. Melissa Lee, Briana Vecchio-Pagán, Neeraj Sharma, Abdul Waheed, Xiaopeng Li, Karen S Raraigh, Sarah Robbins, Sangwoo T Han, Arianna L Franca, Matthew J Pellicore, Taylor A Evans, Kristin M Arcara, Hien Nguyen, Shan Luan, Deborah Belchis, Jozef Hertecant, Joseph Zabner, William S Sly, Garry R Cutting. Human molecular genetics. 2016 May;25:1923-1933. [Full Text Article] [PubMed:26911677] [PMC:PMC5062583]
6
Quantitative Secretome Analysis Reveals Clinical Values of Carbonic Anhydrase II in Hepatocellular Carcinoma. Xiaohua Xing, Hui Yuan, Hongzhi Liu, Xionghong Tan, Bixing Zhao, Yingchao Wang, Jiahe Ouyang, Minjie Lin, Xiaolong Liu, Aimin Huang. Genomics, proteomics & bioinformatics. 2021 Feb;;19:94-107. [Full Text Article] [PubMed:33662630] [PMC:PMC8498920]
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Antibodies (145)
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CA2 / Carbonic Anhydrase II (201)
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CA2 / Carbonic Anhydrase II Antibody - Anti-Carbonic Anhydrase II antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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CA2 / Carbonic Anhydrase II Rabbit anti-Bovine Polyclonal Antibody
Bovine, Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µl/$460
CA2 / Carbonic Anhydrase II Antibody - Anti-Carbonic Anhydrase II antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal (HRP) Antibody
Human
ELISA, IHC, IHC-P, WB
HRP Conjugated
50 µg/$635
CA2 / Carbonic Anhydrase II Antibody - Anti-Carbonic Anhydrase II antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µg/$460
CA2 / Carbonic Anhydrase II Antibody - Fluorescent confocal image of HeLa cell stained with CA2 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CA2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CA2 immunoreactivity is localized to Cytoplasm significantly.
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal (aa54-83) Antibody
Mouse, Human
IF, WB
Unconjugated
400 µl/$393
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Dog
None
10 µg/$288; 50 µg/$487; 100 µg/$780; 200 µg/$972; 1 mg/$2,109; 500 µg/$1,636; 5 mg/$3,403; 2 mg/$2,298
CA2 / Carbonic Anhydrase II ELISA Kit
Select
Sandwich
96-Well Microplate
Human
3.13 - 200 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$651
CA2 / Carbonic Anhydrase II ELISA Kit
Select
Sandwich
96-Well Strip Plate
Human
0.156 - 10 ng/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates
1 Plate/$685
CA2 / Carbonic Anhydrase II Protein
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E. coli
None
Biologically active
100 µg/$411; 500 µg/$985; 20 µg/$276
CA2 / Carbonic Anhydrase II Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal (aa2-260) Antibody
Mouse, Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µg/$460
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CA2 / Carbonic Anhydrase II Rat anti-Human Monoclonal (aa2-260) Antibody
Human
ELISA, WB
Unconjugated
100 µg/$896
CA2 / Carbonic Anhydrase II Antibody - Western blot of recombinant CA2 / Carbonic Anhydrase II.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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CA2 / Carbonic Anhydrase II Rabbit anti-Chicken Polyclonal (aa1-260) Antibody
Chicken, Human
WB
Unconjugated
100 µl/$306; 200 µl/$347; 500 µl/$484; 1 ml/$553; 2 ml/$737; 5 ml/$1,206
CA2 / Carbonic Anhydrase II Antibody - CA2 was detected in paraffin-embedded sections of human gastric cancer tissues using rabbit anti- CA2 Antigen Affinity purified polyclonal antibody
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal Antibody
Human
Flo, ICC, IHC, IHC-P, WB
Unconjugated
10 µg/$318; 100 µg/$470
CA2 / Carbonic Anhydrase II Antibody - Western Blot of Rabbit anti-Carbonic Anhydrase II Antibody Biotin Conjugated. Lane 1: Carbonic Anhydrase II. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carbonic Anhydrase II Antibody Biotin Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT.
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal (Lyophilized) (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated, Lyophilized
100 µg/$552
CA2 / Carbonic Anhydrase II Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
50 µl/$460
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CA2 / Carbonic Anhydrase II Rabbit anti-Mouse Polyclonal Antibody
Mouse
ELISA, IHC, IHC-P, IP, WB
Unconjugated
100 µl/$412
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Human
None
10 µg/$275; 50 µg/$402; 100 µg/$642; 200 µg/$802; 1 mg/$1,876; 500 µg/$1,430; 5 mg/$2,838; 2 mg/$2,060
CA2 / Carbonic Anhydrase II ELISA Kit
Select
Sandwich
96-Well Strip Plate
Chicken
1.56 - 100 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$818
CA2 / Carbonic Anhydrase II Antibody - Western blot analysis of extracts of various cell lines, using CA2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
CA2 / Carbonic Anhydrase II Antibody - 1:100 staining human liver carcinoma tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
2 ml/$1,225
CA2 / Carbonic Anhydrase II Antibody - Western blot of CA II antibody
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal (C-terminal) Antibody
Rat, Human
ELISA, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
CA2 / Carbonic Anhydrase II Antibody - IHC testing of FFPE human gastric cancer tissue with CA2 antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
Flo, IHC-P, WB
Unconjugated
100 µg/$575
CA2 / Carbonic Anhydrase II Antibody - Dot Blot of Rabbit Anti-Carbonic Anhydrase II Peroxidase Conjugated Antibody. Lane 1: 100ng. Lane 2: 33.3ng. Lane 3: 11.1ng. Lane 4: 3.7ng. Lane 5: 1.23ng. Secondary Antibody: Anti-Carbonic Anhydrase II HRP 1µg/mL.
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CA2 / Carbonic Anhydrase II Rabbit anti-Bovine Polyclonal (Lyophilized) (HRP) Antibody
Bovine
DB, ELISA, IHC, WB
HRP Conjugated, Lyophilized
100 µg/$552
CA2 / Carbonic Anhydrase II Antibody - Immunohistochemistry with anti-carbonic anhydrase II antibody showing carbonic anhydrase II staining in nucleus and cytoplasm of proximal and distal tubules, bowman’s capsule and glomerular podocytes of human kidney (B) and in basal cells of retina of a mouse eye (C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-carbonic anhydrase II antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using either MACH 1 universal HRP polymer detection (human kidney) or MACH 4 universal AP polymer detection system (mouse eye) and visualized with 3’3-diamino-benzidine substrate (DAB) or WARP RED.
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal (Liquid) Antibody
Human
ELISA, IHC, WB
Unconjugated, Liquid
25 µl/$304
CA2 / Carbonic Anhydrase II Antibody - Immunohistochemistry with anti-carbonic anhydrase II antibody showing carbonic anhydrase II staining in nucleus and cytoplasm of proximal and distal tubules, bowman’s capsule and glomerular podocytes of human kidney (B) and in basal cells of retina of a mouse eye (C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-carbonic anhydrase II antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using either MACH 1 universal HRP polymer detection (human kidney) or MACH 4 universal AP polymer detection system (mouse eye) and visualized with 3’3-diamino-benzidine substrate (DAB) or WARP RED.
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal (Lyophilized) Antibody
Human
ELISA, IHC, WB
Unconjugated, Lyophilized
100 µg/$567
Viewing 1-25 of 201 product results



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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).