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CA12 / Carbonic Anhydrase XII Antibody LS‑C496894

Western blot analysis of extracts of HT-29 cells, using CA12 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.

CA12 / Carbonic Anhydrase XII Antibody LS‑C496894

Antibody:
Rabbit Polyclonal to Human CA12 / Carbonic Anhydrase XII
Application:
WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$235 
LS-C496894-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human CA12 / Carbonic Anhydrase XII
Application:
WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Carbonic Anhydrase XII antibody LS-C496894 is an unconjugated rabbit polyclonal antibody to Carbonic Anhydrase XII (CA12) from human, mouse and rat. Validated for WB.
Target
Human CA12 / Carbonic Anhydrase XII
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Western blot (1:200 - 1:2000)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 25-301 of human CA12 (NP_001209.1). APVNGSKWTYFGPDGENSWSKKYPSCGGLLQSPIDLHSDILQYDASLTPLEFQGYNLSANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQKFDERLVYTSFSQVQVCTAAGLS
Usage
The predicted MW is 38kDa/39kDa, while the observed MW by Western blot was 50kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CA12 / Carbonic Anhydrase XII
O43570 NM_001218 NP_001209.1

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Flow Cytometry Image
Species: Mouse
Applications: Immunoprecipitation, Flow Cytometry
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRAS.
Species: Human
Applications: IHC - Paraffin, Western blot
Reactivity: Human
Range: 0.94-60 ng/ml
Reactivity: Human
Range: 0.156-10 ng/ml
Reactivity: Rat
Range: 7.8-500 pg/ml

Requested From: United States
Date Requested: 7/19/2019
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