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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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C5AR1 / CD88 / C5a Receptor Antibody (Internal) IHC‑plus™ LS‑A3870
C5a Receptor antibody LS-A3870 is an unconjugated rabbit polyclonal antibody to C5a Receptor (C5AR1 / CD88) from human and monkey. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A3870-50
50 µg (1 mg/ml)
$425
In Stock for Immediate Shipment

Popular C5AR1 / CD88 / C5a Receptor Products

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Antibody
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Product Description

C5a Receptor antibody LS-A3870 is an unconjugated rabbit polyclonal antibody to C5a Receptor (C5AR1 / CD88) from human and monkey. Validated for IHC. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human C5AR1 / CD88 / C5a Receptor
Synonyms
C5AR1, C5A, C5a-R, C5a ligand, C5a receptor, CD88, C5a anaphylatoxin receptor, C5AR, C5R1, CD88 antigen
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 18 amino acid peptide from internal region of human C5AR1 / CD88. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey (100%); Gibbon (89%).
Blocking Peptide
LS-E28831 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A3870. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Internal
Specificity
Human C5AR1 / CD88. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A3870 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A3870 was determined to be 10 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About C5AR1 / CD88 / C5a Receptor
Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. P21730 NM_001736 NP_001727.1


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Immunohistochemistry

Anti-C5AR1 / CD88 antibody LS-A3870 IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-C5AR1 / CD88 antibody LS-A3870 IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-C5AR1 / CD88 antibody LS-A3870 IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-C5AR1 / CD88 antibody LS-A3870 IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-C5AR1 / CD88 antibody LS-A3870 IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-C5AR1 / CD88 antibody LS-A3870 IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-C5AR1 / CD88 antibody LS-A3870 IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-C5AR1 / CD88 antibody LS-A3870 IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-C5AR1 / CD88 antibody LS-A3870 IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-C5AR1 / CD88 antibody LS-A3870 IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-C5AR1 / CD88 antibody LS-A3870 IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-C5AR1 / CD88 antibody LS-A3870 IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-C5AR1 / CD88 antibody LS-A3870 IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-C5AR1 / CD88 antibody LS-A3870 IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-C5AR1 / CD88 antibody LS-A3870 IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-C5AR1 / CD88 antibody LS-A3870 IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: United States
Date Requested: 10/18/2018

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