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C25H / CH25H

Cholesterol 25-hydroxylase

Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.

Gene Name: Cholesterol 25-hydroxylase
Synonyms: CH25H, C25H, Cholesterol 25-hydroxylase, Cholesterol 25-monooxygenase, H25OH
Target Sequences: NM_003956 NP_003947.1 O95992

☰ Filters
Antibodies (9)
Proteins (7)
ELISA Kits (6)
Custom (3)
Over-Expression Lysate (2)
Primary (9)
Recombinant (5)
Sandwich (3)
C25H / CH25H (22)
Human (11)
Mouse (3)
Rat (1)
Chimpanzee (3)
Gibbon (3)
Monkey (3)
Orangutan (3)
Pig (1)
IHC (5)
IHC-P (5)
WB (5)
ELISA (5)
rabbit (9)
Product Group
IHCPlus (1)
Ig (1)
IgG (4)
polyclonal pc (9)
96-Well Strip Plate (6)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
Unconjugated (3)
aa190-239 (4)
N-Terminus (1)
No (22)
Cell Lysates (1)
Plasma (2)
Serum (2)
Tissue Homogenates (2)
GST (1)
GST, N-terminus (2)
His, N-Terminal (1)
Myc-DDK (Flag) (3)
Human (6)
293T Cells (1)
E. coli (1)
HEK 293 Cells (2)
Wheat Germ Extract (3)
Purified (1)
C25H / CH25H Antibody - Anti-C25H / CH25H antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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C25H / CH25H Rabbit anti-Human Polyclonal (aa190-239) Antibody
Human
IHC, IHC-P, WB
Unconjugated
50 µl/$460
C25H / CH25H Antibody - Anti-C25H / CH25H antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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C25H / CH25H Rabbit anti-Human Polyclonal (aa190-239) (HRP) Antibody
Human, Monkey, Chimpanzee, Orangutan, Gibbon
IHC, IHC-P, WB
HRP Conjugated
100 µl/$469
C25H / CH25H Antibody - Anti-C25H / CH25H antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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C25H / CH25H Rabbit anti-Human Polyclonal (aa190-239) (Biotin) Antibody
Human, Monkey, Chimpanzee, Orangutan, Gibbon
IHC, IHC-P, WB
Biotin Conjugated
100 µl/$469
C25H / CH25H Antibody - Anti-C25H / CH25H antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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C25H / CH25H Rabbit anti-Human Polyclonal (aa190-239) (FITC) Antibody
Human, Monkey, Chimpanzee, Orangutan, Gibbon
IHC, IHC-P, WB
FITC Conjugated
100 µl/$469
C25H / CH25H Antibody - All lanes: Anti-CH25H Antibody (N-Term) at 1:1000-1:2000 dilution Lane 1: Human brain lysate Lane 2: Mouse heart lysate Lane 3: Mouse lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 32 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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C25H / CH25H Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Mouse, Human
ELISA, WB
Unconjugated
200 µl/$393
C25H / CH25H ELISA Kit
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Sandwich
96-Well Strip Plate
Mouse
0.781 - 50 ng/ml
Colorimetric - 450nm (TMB)
Cell Lysates, Tissue Homogenates
1 Plate/$854
C25H / CH25H ELISA Kit
Select
Sandwich
96-Well Strip Plate
Human
125 - 8000 pg/ml
Colorimetric - 450nm (TMB)
Plasma, Serum, Tissue Homogenates
1 Plate/$834
C25H / CH25H Protein - Recombinant Cholesterol-25-Hydroxylase (CH25H) by SDS-PAGE
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E. coli
His, N-Terminal
16.4kDa
10 µg/$267; 50 µg/$352; 200 µg/$702; 100 µg/$562; 1 mg/$1,723; 500 µg/$1,298; 5 mg/$2,673; 2 mg/$1,900
C25H / CH25H Protein - Purified recombinant protein CH25H was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
31.6 kDa
20 µg/$1,107
C25H / CH25H Antibody - Immunohistochemistry of paraffin-embedded human colon cancer at dilution of 1:100
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C25H / CH25H Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P
Unconjugated
50 µg/$294; 100 µg/$360
Select
C25H / CH25H Rabbit anti-Human Polyclonal (HRP) Antibody
Human
ELISA
HRP Conjugated
50 µg/$294; 100 µg/$360
Select
C25H / CH25H Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
ELISA
Biotin Conjugated
50 µg/$294; 100 µg/$360
Select
C25H / CH25H Rabbit anti-Human Polyclonal (FITC) Antibody
Human
ELISA
FITC Conjugated
50 µg/$294; 100 µg/$360
C25H / CH25H Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
C25H / CH25H Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
C25H / CH25H ELISA Kit
Select
Custom
96-Well Strip Plate
Mouse
1 Plate/$783
C25H / CH25H ELISA Kit
Select
Sandwich
96-Well Strip Plate
Human
0.156 - 10 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$783
C25H / CH25H ELISA Kit
Select
Custom
96-Well Strip Plate
Rat
1 Plate/$783
C25H / CH25H ELISA Kit
Select
Custom
96-Well Strip Plate
Pig
1 Plate/$783
C25H / CH25H Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
31.6 kDa
20 µg/$150
C25H / CH25H Protein - 12.5% SDS-PAGE of human CH25H stained with Coomassie Blue
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Wheat Germ Extract
GST
58.1 kDa
10 µg/$479; 25 µg/$670
C25H / CH25H Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
31.6 kDa
100 µg/$494
Viewing 1-22 of 22 product results



If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).