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Catalog Number Size Price
LS-C745305-25 25 µl (1.01 mg/ml) $275 
c-Met Antibody - Western blot using affinity purified anti-c-Met pY1349pY1356 antibody shows detection of phosphorylated c-Met. Human mammary epithelial cells (B5/589) were serum deprived and stimulated with (+) and without (-) HGF. Cell lysates were immunoprecipitated using a human anti-c-Met antibody, resolved by SDS-PAGE, transferred to PVDF membrane and probed using various antibodies. Lane 1 and 2 where probed using the anti-c-Met pY1349pY1356 antibody, lane 3 and 4 where probed using an anti-phosphotyrosine antibody as phosphorylation control and lane 5 and 6 where probed using an anti-cMet antibody as a total Met loading control. Bands recognized at an apparent molecular weight of ~170 kDa are total Met, ~145 kDa phosphorylated Met beta chain in HGF +, ~150 kDa phosphorylated Met in +/- HGF, ~50kDa phosphorylated Met alpha chain.
c-Met Antibody - Western blot using the affinity purified anti-c-Met pY1349pY1356 antibody shows detection of phosphorylated c-Met. Human mammary B5/589 epithelial cells were serum-deprived and treated with or without HGF. Cell lysates were immunoprecipated with the anti-c-Met antibody, resolved by SDS-PAGE, transferred to PVDF membrane, and probed with anti-c-Met pY1349pY1356.
c-Met Antibody - Western blot using affinity purified anti-c-Met pY1349pY1356 antibody shows detection of phosphorylated c-Met. Human mammary epithelial cells (B5/589) were serum deprived and stimulated with (+) and without (-) HGF. Cell lysates were immunoprecipitated using a human anti-c-Met antibody, resolved by SDS-PAGE, transferred to PVDF membrane and probed using various antibodies. Lane 1 and 2 where probed using the anti-c-Met pY1349pY1356 antibody, lane 3 and 4 where probed using an anti-phosphotyrosine antibody as phosphorylation control and lane 5 and 6 where probed using an anti-cMet antibody as a total Met loading control. Bands recognized at an apparent molecular weight of ~170 kDa are total Met, ~145 kDa phosphorylated Met beta chain in HGF +, ~150 kDa phosphorylated Met in +/- HGF, ~50kDa phosphorylated Met alpha chain.
c-Met Antibody - Western blot using the affinity purified anti-c-Met pY1349pY1356 antibody shows detection of phosphorylated c-Met. Human mammary B5/589 epithelial cells were serum-deprived and treated with or without HGF. Cell lysates were immunoprecipated with the anti-c-Met antibody, resolved by SDS-PAGE, transferred to PVDF membrane, and probed with anti-c-Met pY1349pY1356.
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Polyclonal Rabbit anti‑Human c‑Met Antibody (phospho‑Tyr1349/1356, WB) LS‑C745305

Polyclonal Rabbit anti‑Human c‑Met Antibody (phospho‑Tyr1349/1356, WB) LS‑C745305

Antibody:
c-Met Rabbit anti-Human Polyclonal (pTyr1349/1356) Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745305-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
c-Met Rabbit anti-Human Polyclonal (pTyr1349/1356) Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
C-Met antibody LS-C745305 is an unconjugated rabbit polyclonal antibody to c-Met (pTyr1349/1356) from human. It is reactive with human, mouse and rat. Validated for ELISA and WB.
Target
Human c-Met
Synonyms
MET | AUTS9 | C-Met | HGF/SF receptor | HGFR | Met kinase | Proto-oncogene c-Met | Scatter factor receptor | SF receptor | HGF receptor | RCCP2 | Tyrosine-protein kinase Met
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y1349 and Y1356 of human c-Met protein.
Epitope
pTyr1349/1356
Specificity
This antibody is specific for human c-Met protein phosphorylated at Y1349 and Y1356. A BLAST analysis was used to suggest cross-reactivity with c-Met from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with c-Met from other sources has not been determined.
Applications
  • Western blot (1 µg/ml)
  • ELISA (1:93000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About c-Met
P08581 NM_000245 NP_000236.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/13/2021