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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C4340-200 200 µg $605 
Antibody

c‑CBL Antibody (aa878‑906) LS‑C4340

c‑CBL Antibody (aa878‑906) LS‑C4340

Antibody:
Mouse Monoclonal to Human c-CBL
Application:
ICC, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$605 
LS-C4340-200
200 µg
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human c-CBL
Application:
ICC, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
C-CBL antibody LS-C4340 is an unconjugated mouse monoclonal antibody to c-CBL from human, mouse and rat. Validated for ICC, IP and WB.
Target
Human c-CBL
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • ICC (5 - 10 µg/ml)
  • Western blot
  • Immunoprecipitation
  • Applications tested for the base form of this product only
Immunogen
GST fusion protein corresponding to residues 878-906 of human Cbl. This sequence is identical in mouse.
Epitope
aa878-906
Specificity
Specific for Cbl. Species cross-reactivity: mouse and rat.
Usage
Suitable for use in Western Blot, Immunoprecipitation, and Immunocytochemistry. Western Blot Analysis: 0.5-2 ug/ml detects Cbl (120kD) in RIPA lysates from human Jurkat and mouse 3T3 cells. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Cbl (1 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 2 ug of a previous lot immunoprecipitated Cbl from 1 ug of a mouse 3T3 cell RIPA lysate. Immunocytochemistry: 5-10 ug/ml of a previous lot showed positive immunostaining for Cbl in 3T3 cells fixed with ice cold methanol/ethanol [1:1] for 1 minute. Positive control: F4209:3T3 lysate. Use 20 ug per lane for minigels.
Presentation
PBS, 0.05% Sodium Azide, 20% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About c-CBL
P22681 NM_005188 NP_005179.2

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Requested From: United States
Date Requested: 11/18/2019