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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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C/EBP Delta / CEBPD Antibody (N‑Terminus) LS‑C60129
CEBPD antibody LS-C60129 is an unconjugated rabbit polyclonal antibody to CEBPD (C / EBP Delta) from human and mouse. Validated for ELISA and WB.
Catalog
Size
Price
LS-C60129-100
100 µg (1.33 mg/ml)
$425
Description
CEBPD antibody LS-C60129 is an unconjugated rabbit polyclonal antibody to CEBPD (C / EBP Delta) from human and mouse. Validated for ELISA and WB.
Target
Mouse C/EBP Delta / CEBPD
Host
Rabbit
Reactivity
Mouse, Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:1000 - 1:15000)
  • ELISA (1:4000 - 1:9000)
Immunogen
Recombinant protein corresponding to the amino terminus of mouse C/EBP.
Epitope
N-Terminus
Specificity
This antibody recognizes endogenous mouse and recombinant human C/EBP delta protein. A BLAST analysis was used to suggest cross-reactivity with C/EBP delta from human sources based on 77% homology with the immunizing sequence. No cross-reactivity is observed with mouse C/EBP alpha or C/EBP beta. Reactivity with C/EBP delta from other sources has not been determined
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 35 kD in size corresponding to C/EBPS by western blotting in the appropriate cell lysate or extract. The predicted molecular weight of mouse C/EBPS is 28.8 kD. This antibody is capable of detecting both over-expressed and endogenous C/EBPS.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only.
About C/EBP Delta / CEBPD
P49716 NM_005195 NP_005186.2

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Western Blot of Anti-C/EBPd Antibody Lane 1: untreated wild-type (WT) cells Lane 2: WT cells treated 3 h with LPS Lane 3: C/EBPd-knock-out cells treated 3H with LPS Load: 35 µg per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4°C Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kDa, ~35 kDa
Species: Mouse, Human
Applications: IHC, Western blot, Immunoprecipitation, ELISA

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Images

Western blot

Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: untreated wild-type (WT) cells Lane 2: WT cells treated 3 h with LPS Lane 3: C/EBPd-knock-out cells treated 3H with LPS Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kD, ~35 kD.
Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: untreated wild-type (WT) cells Lane 2: WT cells treated 3 h with LPS Lane 3: C/EBPd-knock-out cells treated 3H with LPS Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kD, ~35 kD.

Western blot

Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: human C/EBPbeta Lane 2: Mouse C/EBPdelta Lane 3: untransfected Lane 4: human C/EBPdelta Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kD, ~35 kD.
Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: human C/EBPbeta Lane 2: Mouse C/EBPdelta Lane 3: untransfected Lane 4: human C/EBPdelta Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kD, ~35 kD.

Western blot

Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: untreated wild-type (WT) cells Lane 2: WT cells treated 3 h with LPS Lane 3: C/EBPd-knock-out cells treated 3H with LPS Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kD, ~35 kD.
Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: untreated wild-type (WT) cells Lane 2: WT cells treated 3 h with LPS Lane 3: C/EBPd-knock-out cells treated 3H with LPS Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kD, ~35 kD.

Western blot

Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: human C/EBPbeta Lane 2: Mouse C/EBPdelta Lane 3: untransfected Lane 4: human C/EBPdelta Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kD, ~35 kD.
Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: human C/EBPbeta Lane 2: Mouse C/EBPdelta Lane 3: untransfected Lane 4: human C/EBPdelta Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kD, ~35 kD.

Western blot

Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: untreated wild-type (WT) cells Lane 2: WT cells treated 3 h with LPS Lane 3: C/EBPd-knock-out cells treated 3H with LPS Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kD, ~35 kD.
Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: untreated wild-type (WT) cells Lane 2: WT cells treated 3 h with LPS Lane 3: C/EBPd-knock-out cells treated 3H with LPS Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kD, ~35 kD.

Western blot

Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: human C/EBPbeta Lane 2: Mouse C/EBPdelta Lane 3: untransfected Lane 4: human C/EBPdelta Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kD, ~35 kD.
Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: human C/EBPbeta Lane 2: Mouse C/EBPdelta Lane 3: untransfected Lane 4: human C/EBPdelta Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kD, ~35 kD.

Western blot

Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: untreated wild-type (WT) cells Lane 2: WT cells treated 3 h with LPS Lane 3: C/EBPd-knock-out cells treated 3H with LPS Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kD, ~35 kD.
Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: untreated wild-type (WT) cells Lane 2: WT cells treated 3 h with LPS Lane 3: C/EBPd-knock-out cells treated 3H with LPS Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kD, ~35 kD.

Western blot

Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: human C/EBPbeta Lane 2: Mouse C/EBPdelta Lane 3: untransfected Lane 4: human C/EBPdelta Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kD, ~35 kD.
Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: human C/EBPbeta Lane 2: Mouse C/EBPdelta Lane 3: untransfected Lane 4: human C/EBPdelta Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kD, ~35 kD.

Requested From: United States
Date Requested: 12/13/2018
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