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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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C/EBP Delta / CEBPD Antibody (C‑Terminus) IHC‑plus™ LS‑B10190 Best Seller
Note: This antibody replaces LS-C153725
CEBPD antibody LS-B10190 is an unconjugated rabbit polyclonal antibody to CEBPD (C / EBP Delta) from human, mouse and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B10190-50
50 µg (1 mg/ml)
$425

Popular C/EBP Delta / CEBPD Products

Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: untreated wild-type (WT) cells Lane 2: WT cells treated 3 h with LPS Lane 3: C/EBPd-knock-out cells treated 3H with LPS Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kD, ~35 kD.
Species: Mouse, Human
Applications: Western blot, ELISA
Immunohistochemistry of Human colon cancer using CEBPD Polyclonal Antibody at dilution of 1:40.
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA
Western blot analysis of Mouse lung tissue, using CEBPD Polyclonal Antibody at dilution of 1:250.
Species: Human, Mouse, Rat
Applications: Western blot, ELISA
Western Blot of Anti-C/EBPd Antibody Lane 1: untreated wild-type (WT) cells Lane 2: WT cells treated 3 h with LPS Lane 3: C/EBPd-knock-out cells treated 3H with LPS Load: 35 µg per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4°C Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kDa, ~35 kDa
Species: Mouse, Human
Applications: IHC, Western blot, Immunoprecipitation, ELISA

Product Description

C/EBP Delta / CEBPD Antibody (C-Terminus) for IHC, IF/Immunofluorescence, WB/Western, ELISA IHC-plus™ LS-B10190

Specifications

Target
Human C/EBP Delta / CEBPD
Synonyms
CEBPD, C/EBP-delta, C/EBP delta, CELF, CRP3, NF-IL6-beta, Nuclear factor NF-IL6-beta
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (20 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E31351 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B10190. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Multiple transcript variants encoding different isoforms have been found for this gene.
Usage
CEBPD antibody can be used for detection of CEBPD by Western blot at 0.5-1 ug/mL.
Presentation
PBS, 0.02% Sodium Azide
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
About C/EBP Delta / CEBPD
C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses, may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages. P49716 NM_005195 NP_005186.2


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Images

Immunohistochemistry

Anti-C/EBP Delta / CEBPD antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10190 concentration 10 ug/ml.
Anti-C/EBP Delta / CEBPD antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10190 concentration 10 ug/ml.

Immunohistochemistry

Anti-C/EBP Delta / CEBPD antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-C/EBP Delta / CEBPD antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunofluorescence

Immunofluorescence of CEBPD in human spleen tissue with CEBPD antibody at 20 µg/mL.
Immunofluorescence of CEBPD in human spleen tissue with CEBPD antibody at 20 µg/mL.

Western blot

Western blot analysis of CEBPD in rat spleen tissue lysate with CEBPD antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of CEBPD in rat spleen tissue lysate with CEBPD antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry

Anti-C/EBP Delta / CEBPD antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10190 concentration 10 ug/ml.
Anti-C/EBP Delta / CEBPD antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10190 concentration 10 ug/ml.

Immunohistochemistry

Anti-C/EBP Delta / CEBPD antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-C/EBP Delta / CEBPD antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunofluorescence

Immunofluorescence of CEBPD in human spleen tissue with CEBPD antibody at 20 µg/mL.
Immunofluorescence of CEBPD in human spleen tissue with CEBPD antibody at 20 µg/mL.

Western blot

Western blot analysis of CEBPD in rat spleen tissue lysate with CEBPD antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of CEBPD in rat spleen tissue lysate with CEBPD antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry

Anti-C/EBP Delta / CEBPD antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10190 concentration 10 ug/ml.
Anti-C/EBP Delta / CEBPD antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10190 concentration 10 ug/ml.

Immunohistochemistry

Anti-C/EBP Delta / CEBPD antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-C/EBP Delta / CEBPD antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunofluorescence

Immunofluorescence of CEBPD in human spleen tissue with CEBPD antibody at 20 µg/mL.
Immunofluorescence of CEBPD in human spleen tissue with CEBPD antibody at 20 µg/mL.

Western blot

Western blot analysis of CEBPD in rat spleen tissue lysate with CEBPD antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of CEBPD in rat spleen tissue lysate with CEBPD antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry

Anti-C/EBP Delta / CEBPD antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10190 concentration 10 ug/ml.
Anti-C/EBP Delta / CEBPD antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10190 concentration 10 ug/ml.

Immunohistochemistry

Anti-C/EBP Delta / CEBPD antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-C/EBP Delta / CEBPD antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunofluorescence

Immunofluorescence of CEBPD in human spleen tissue with CEBPD antibody at 20 µg/mL.
Immunofluorescence of CEBPD in human spleen tissue with CEBPD antibody at 20 µg/mL.

Western blot

Western blot analysis of CEBPD in rat spleen tissue lysate with CEBPD antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of CEBPD in rat spleen tissue lysate with CEBPD antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Requested From: United States
Date Requested: 10/20/2018

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