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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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C/EBP Alpha / CEBPA Antibody (aa342‑358) LS‑C312618
Note: This antibody replaces LS-C388246
CEBPA antibody LS-C312618 is an unconjugated rabbit polyclonal antibody to CEBPA (C / EBP Alpha) from human, mouse, rat and other species. Validated for ICC, IHC and WB.
Catalog
Size
Price
LS-C312618-100
100 µg (0.5 mg/ml)
$355

Popular C/EBP Alpha / CEBPA Products

Anti-CEBPA / CEBP Alpha antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4685 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Pig, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Mouse
Applications: Western blot, Flow Cytometry, ELISA
Antibody
Species: Human, Mouse
Applications: Western blot, Flow Cytometry, ELISA
CEBPA / CEBP Alpha antibody LS-C117063 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Guinea pig, Pig, Zebrafish
Applications: Western blot
Western blot of Phospho-C/EBP alpha (S21) antibody
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA

Product Description

CEBPA antibody LS-C312618 is an unconjugated rabbit polyclonal antibody to CEBPA (C / EBP Alpha) from human, mouse, rat and other species. Validated for ICC, IHC and WB.
About C/EBP Alpha / CEBPA
This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. P49715 NM_004364 NP_004355.2

CEBPA Antibody, C/EBP alpha Antibody, C/EBP-alpha Antibody, CEBP Antibody


Specifications

Target
Human C/EBP Alpha / CEBPA
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Pig, Rabbit, Sheep, Xenopus (tested or 100% immunogen sequence identity)
Predicted
Hamster (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CEBP Alpha(342-358aa FRQLPESSLVKAMGNCA), identical to the related mouse and rat sequences.
Epitope
aa342-358
Presentation
Contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2 HPO4, 0.05 mg Thimerosal, 0.05 mg sodium azide per 100 ug antibody.
Reconstitution
200 µl Distilled water
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry

C/EBP Alpha / CEBPA antibody. IHC(P): Human Breast Cancer Tissue.
C/EBP Alpha / CEBPA antibody. IHC(P): Human Breast Cancer Tissue.

Western blot

WB of C/EBP Alpha / CEBPA antibody. All lanes: Anti-CEBPA at 0.5ug/ml. Lane 1: MM231 Whole Cell Lysate at 40ug. Lane 2: JURKAT Whole Cell Lysate at 40ug. Lane 3: RAJI Whole Cell Lysate at 40ug. Lane 4: HELA Whole Cell Lysate at 40ug. Predicted bind size: 37KD. Observed bind size: 37KD.
WB of C/EBP Alpha / CEBPA antibody. All lanes: Anti-CEBPA at 0.5ug/ml. Lane 1: MM231 Whole Cell Lysate at 40ug. Lane 2: JURKAT Whole Cell Lysate at 40ug. Lane 3: RAJI Whole Cell Lysate at 40ug. Lane 4: HELA Whole Cell Lysate at 40ug. Predicted bind size: 37KD. Observed bind size: 37KD.

Immunohistochemistry

C/EBP Alpha / CEBPA antibody. IHC(P): Human Breast Cancer Tissue.
C/EBP Alpha / CEBPA antibody. IHC(P): Human Breast Cancer Tissue.

Western blot

WB of C/EBP Alpha / CEBPA antibody. All lanes: Anti-CEBPA at 0.5ug/ml. Lane 1: MM231 Whole Cell Lysate at 40ug. Lane 2: JURKAT Whole Cell Lysate at 40ug. Lane 3: RAJI Whole Cell Lysate at 40ug. Lane 4: HELA Whole Cell Lysate at 40ug. Predicted bind size: 37KD. Observed bind size: 37KD.
WB of C/EBP Alpha / CEBPA antibody. All lanes: Anti-CEBPA at 0.5ug/ml. Lane 1: MM231 Whole Cell Lysate at 40ug. Lane 2: JURKAT Whole Cell Lysate at 40ug. Lane 3: RAJI Whole Cell Lysate at 40ug. Lane 4: HELA Whole Cell Lysate at 40ug. Predicted bind size: 37KD. Observed bind size: 37KD.

Immunohistochemistry

C/EBP Alpha / CEBPA antibody. IHC(P): Human Breast Cancer Tissue.
C/EBP Alpha / CEBPA antibody. IHC(P): Human Breast Cancer Tissue.

Western blot

WB of C/EBP Alpha / CEBPA antibody. All lanes: Anti-CEBPA at 0.5ug/ml. Lane 1: MM231 Whole Cell Lysate at 40ug. Lane 2: JURKAT Whole Cell Lysate at 40ug. Lane 3: RAJI Whole Cell Lysate at 40ug. Lane 4: HELA Whole Cell Lysate at 40ug. Predicted bind size: 37KD. Observed bind size: 37KD.
WB of C/EBP Alpha / CEBPA antibody. All lanes: Anti-CEBPA at 0.5ug/ml. Lane 1: MM231 Whole Cell Lysate at 40ug. Lane 2: JURKAT Whole Cell Lysate at 40ug. Lane 3: RAJI Whole Cell Lysate at 40ug. Lane 4: HELA Whole Cell Lysate at 40ug. Predicted bind size: 37KD. Observed bind size: 37KD.

Immunohistochemistry

C/EBP Alpha / CEBPA antibody. IHC(P): Human Breast Cancer Tissue.
C/EBP Alpha / CEBPA antibody. IHC(P): Human Breast Cancer Tissue.

Western blot

WB of C/EBP Alpha / CEBPA antibody. All lanes: Anti-CEBPA at 0.5ug/ml. Lane 1: MM231 Whole Cell Lysate at 40ug. Lane 2: JURKAT Whole Cell Lysate at 40ug. Lane 3: RAJI Whole Cell Lysate at 40ug. Lane 4: HELA Whole Cell Lysate at 40ug. Predicted bind size: 37KD. Observed bind size: 37KD.
WB of C/EBP Alpha / CEBPA antibody. All lanes: Anti-CEBPA at 0.5ug/ml. Lane 1: MM231 Whole Cell Lysate at 40ug. Lane 2: JURKAT Whole Cell Lysate at 40ug. Lane 3: RAJI Whole Cell Lysate at 40ug. Lane 4: HELA Whole Cell Lysate at 40ug. Predicted bind size: 37KD. Observed bind size: 37KD.

Requested From: United States
Date Requested: 9/24/2018

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