BubR1 antibody LS-C18942 is an unconjugated mouse monoclonal antibody to human BubR1 (BUB1B). Validated for IF, IP and WB.
Human BUB1B / BubR1
(tested or 100% immunogen sequence identity)
Protein A purified
- Western blot
BUB1B / BubR1 antibody was raised against recombinant human BUB1B / BubR1.
Amino acid residues 1-350 of human BUBR1 Kinase protein.
This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~120 kD corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. Higher MW bands may be seen that may be due to hyper-phosphorylation of the protein. The use of HeLa whole cell lysates is recommended as a positive control. For IF microscopy use cells grown on cover slips fixed with 3.5% paraformaldehyde in PBS at pH 6.8. Permeabilize fixed cells with 0.2% Triton X-100 in 25 mM Tris Cl, pH 7.4 containing 0.1% BSA.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.