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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BUB1B / BubR1 Antibody (aa1-350) LS-C18942

BubR1 antibody LS-C18942 is an unconjugated mouse monoclonal antibody to human BubR1 (BUB1B). Validated for IF, IP and WB.
Catalog
Size
Price
LS-C18942-100
100 µg (1 mg/ml)
$425
Description
BubR1 antibody LS-C18942 is an unconjugated mouse monoclonal antibody to human BubR1 (BUB1B). Validated for IF, IP and WB.
Target
Human BUB1B / BubR1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
Failed Applications
  • IHC - Paraffin
Immunogen
BUB1B / BubR1 antibody was raised against recombinant human BUB1B / BubR1.
Epitope
aa1-350
Specificity
Amino acid residues 1-350 of human BUBR1 Kinase protein.
Usage
This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~120 kD corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. Higher MW bands may be seen that may be due to hyper-phosphorylation of the protein. The use of HeLa whole cell lysates is recommended as a positive control. For IF microscopy use cells grown on cover slips fixed with 3.5% paraformaldehyde in PBS at pH 6.8. Permeabilize fixed cells with 0.2% Triton X-100 in 25 mM Tris Cl, pH 7.4 containing 0.1% BSA.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About BUB1B / BubR1
O60566 NM_001211 NP_001202.4

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Requested From: United States
Date Requested: 4/21/2019
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