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Catalog Number Size Price
LS-C18942-100 100 µg (1 mg/ml) $498 
Antibody

Monoclonal Mouse anti‑Human BUB1B / BubR1 Antibody (clone 8G1, aa1‑350, IF, WB) LS‑C18942

Monoclonal Mouse anti‑Human BUB1B / BubR1 Antibody (clone 8G1, aa1‑350, IF, WB) LS‑C18942

Antibody:
BUB1B / BubR1 Mouse anti-Human Monoclonal (aa1-350) (8G1) Antibody
Application:
IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$498
LS-C18942-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
BUB1B / BubR1 Mouse anti-Human Monoclonal (aa1-350) (8G1) Antibody
Application:
IF, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
BubR1 antibody LS-C18942 is an unconjugated mouse monoclonal antibody to human BubR1 (BUB1B) (aa1-350). Validated for ELISA, IF, IP and WB.
Target
Human BUB1B / BubR1
Synonyms
BUB1B | Bub1A | BUBR1 | HBUBR1 | Protein SSK1 | SSK1 | BUB1beta | MAD3L
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
8G1
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 1-350 of human BUBR1 Kinase protein.
Epitope
aa1-350
Applications
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:200 - 1:1000)
  • Immunoprecipitation (1:100)
  • ELISA (1:5000 - 1:20000)
Usage
This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~120 kD corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. Higher MW bands may be seen that may be due to hyper-phosphorylation of the protein. The use of HeLa whole cell lysates is recommended as a positive control. For IF microscopy use cells grown on cover slips fixed with 3.5% paraformaldehyde in PBS at pH 6.8. Permeabilize fixed cells with 0.2% Triton X-100 in 25 mM Tris Cl, pH 7.4 containing 0.1% BSA.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BUB1B / BubR1
O60566 NM_001211 NP_001202.4

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/15/2022