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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BUB1 Antibody (aa281-419) LS-C18935

BUB1 antibody LS-C18935 is an unconjugated mouse monoclonal antibody to human BUB1. Validated for IF, IP and WB.
Catalog
Size
Price
LS-C18935-100
100 µg (1 mg/ml)
$425
Description
BUB1 antibody LS-C18935 is an unconjugated mouse monoclonal antibody to human BUB1. Validated for IF, IP and WB.
Target
Human BUB1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
Immunogen
BUB1 antibody was raised against recombinant human Bub1.
Epitope
aa281-419
Specificity
Amino acid residues 281-419 of human BUB1 protein.
Usage
This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~160 kD corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. The use of HeLa whole cell lysates prepared using a RIPA buffer is recommended as a positive control. For IF microscopy use cells grown on cover slips fixed with 3.5% paraformaldehyde in PBS at pH 6.8 OR 100% methanol at -20? C. Permeabilize fixed cells with 0.5% Triton X-100.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About BUB1
O43683 NM_004336 NP_004327.1

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Requested From: United States
Date Requested: 4/21/2019
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