BUB1 antibody LS-C18935 is an unconjugated mouse monoclonal antibody to human BUB1. Validated for IF, IP and WB.
(tested or 100% immunogen sequence identity)
Protein A purified
- Western blot
BUB1 antibody was raised against recombinant human Bub1.
Amino acid residues 281-419 of human BUB1 protein.
This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~160 kD corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. The use of HeLa whole cell lysates prepared using a RIPA buffer is recommended as a positive control. For IF microscopy use cells grown on cover slips fixed with 3.5% paraformaldehyde in PBS at pH 6.8 OR 100% methanol at -20? C. Permeabilize fixed cells with 0.5% Triton X-100.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
For research use only.