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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BRAF / B-Raf Antibody (phospho-Thr598/Ser601) IHC-plus™ LS-B1627

Note: This antibody replaces LS-C41172
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Overnight nocodazole treated Hela cells stained with purified rabbit polyclonal antibody against Thr598/Ser601 phosphorylated B-Raf, followed by Rhodamine Red-X conjugated goat anti-rabbit IgG and DAPI.
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Overnight nocodazole treated Hela cells stained with purified rabbit polyclonal antibody against Thr598/Ser601 phosphorylated B-Raf, followed by Rhodamine Red-X conjugated goat anti-rabbit IgG and DAPI.
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BRAF / B-Raf Antibody (phospho-Thr598/Ser601) IHC-plus™ LS-B1627

Note: This antibody replaces LS-C41172
Antibody:
Rabbit Polyclonal to Human BRAF / B-Raf
Application:
IHC, IHC-P, ICC, IF
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B1627-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human BRAF / B-Raf
Application:
IHC, IHC-P, ICC, IF
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
B-Raf antibody LS-B1627 is an unconjugated rabbit polyclonal antibody to human B-Raf (BRAF). Validated for ICC, IF and IHC. Tested on 20 paraffin-embedded human tissues.

Target
Human BRAF / B-Raf
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • ICC
  • Immunofluorescence
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
BRAF / B-Raf antibody was raised against synthetic peptide from human BRAF.
Epitope
pThr598/Ser601
Specificity
Modified peptide
Usage
Immunohistochemistry: LS-B1627 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1627 was determined to be 1:100.
Presentation
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 50% glycerol.
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BRAF / B-Raf
P15056 NM_004333 NP_004324.2

Validation

Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
IHC Validated
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRAS.
Species: Human
Applications: Western blot
Reactivity: Rat
Range: 0.188-12 ng/ml
Reactivity: Human
Range: 0.156-10 ng/ml
Reactivity: Human
Range: 0.156-10 ng/ml

Requested From: United States
Date Requested: 6/17/2019
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