B-Raf antibody LS-B1627 is an unconjugated rabbit polyclonal antibody to human B-Raf (BRAF). Validated for ICC, IF and IHC. Tested on 20 paraffin-embedded human tissues.
Human BRAF / B-Raf
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (1:100)
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BRAF / B-Raf antibody was raised against synthetic peptide from human BRAF.
Immunohistochemistry: LS-B1627 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1627 was determined to be 1:100.
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 50% glycerol.
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.