B-Raf antibody LS-B10112 is an unconjugated rabbit polyclonal antibody to B-Raf (BRAF) from human, mouse and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Human BRAF / B-Raf
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Peptide Column Affinity Chromatography
- IHC - Paraffin (10 µg/ml)
- Immunofluorescence (20 µg/ml)
- Western blot (1 - 2 µg/ml)
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BRAF / B-Raf antibody was raised against a 19 amino acid peptide near the center of human B-raf. The immunogen is located within amino acids 330 - 380 of B-raf.
- Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-B10112. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
At least two isoforms of B-raf are known to exist; this antibody will detect both isoforms. This antibody will not cross-react with C-raf.
B-raf antibody can be used for detection of B-raf by Western blot at 1 and 2 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 84 Observed: 99, multiple post-translational modifications, commonly observed at 95-100kDa
PBS containing 0.02% Sodium Azide
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
For research use only.