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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BPI Antibody LS‑C498452
BPI antibody LS-C498452 is an unconjugated rabbit polyclonal antibody to human BPI. Validated for ELISA, IF and WB.
Catalog
Size
Price
LS-C498452-50
50 µg
$285
LS-C498452-100
100 µg
$385
Description
BPI antibody LS-C498452 is an unconjugated rabbit polyclonal antibody to human BPI. Validated for ELISA, IF and WB.
Target
Human BPI
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with HRP, Biotin, FITC.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot
  • ELISA
  • (applications tested for the base form of this product only)
Immunogen
BPI antibody was raised against recombinant Human Bactericidal permeability-increasing protein (149-362AA)
Specificity
The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa.
Presentation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Storage
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Restrictions
For research use only.
About BPI
P17213 NM_001725 NP_001716.2

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Immunofluorescent analysis of BPI staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Species: Human, Mouse
Applications: ICC, Immunofluorescence, Western blot

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Images

Immunofluorescence

Immunofluorescent analysis of HepG2 cells using BPI Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of HepG2 cells using BPI Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Western blot

Positive WB detected in:HEK293 whole cell lysate,THP-1 whole cell lysate;All lanes:BPI antibody at 4ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 54 kDa;Observed band size: 54 kDa;
Positive WB detected in:HEK293 whole cell lysate,THP-1 whole cell lysate;All lanes:BPI antibody at 4ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 54 kDa;Observed band size: 54 kDa;

Immunofluorescence

Immunofluorescent analysis of HepG2 cells using BPI Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of HepG2 cells using BPI Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Western blot

Positive WB detected in:HEK293 whole cell lysate,THP-1 whole cell lysate;All lanes:BPI antibody at 4ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 54 kDa;Observed band size: 54 kDa;
Positive WB detected in:HEK293 whole cell lysate,THP-1 whole cell lysate;All lanes:BPI antibody at 4ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 54 kDa;Observed band size: 54 kDa;

Immunofluorescence

Immunofluorescent analysis of HepG2 cells using BPI Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of HepG2 cells using BPI Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Western blot

Positive WB detected in:HEK293 whole cell lysate,THP-1 whole cell lysate;All lanes:BPI antibody at 4ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 54 kDa;Observed band size: 54 kDa;
Positive WB detected in:HEK293 whole cell lysate,THP-1 whole cell lysate;All lanes:BPI antibody at 4ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 54 kDa;Observed band size: 54 kDa;

Immunofluorescence

Immunofluorescent analysis of HepG2 cells using BPI Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of HepG2 cells using BPI Antibody at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Western blot

Positive WB detected in:HEK293 whole cell lysate,THP-1 whole cell lysate;All lanes:BPI antibody at 4ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 54 kDa;Observed band size: 54 kDa;
Positive WB detected in:HEK293 whole cell lysate,THP-1 whole cell lysate;All lanes:BPI antibody at 4ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 54 kDa;Observed band size: 54 kDa;

Requested From: United States
Date Requested: 12/18/2018
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