BLM antibody LS-C209389 is an unconjugated rabbit polyclonal antibody to human BLM. Validated for ELISA, IHC and WB.
(tested or 100% immunogen sequence identity)
- Western blot (1 µg/ml)
- ELISA (1:10000)
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BLM antibody was raised against anti-BLM pS186 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human Bloom syndrome protein.
The antibody is directed against the phosphorylated form of human S186 residue. A BLAST analysis was used to suggest cross-reactivity with human based on 100% sequence homology. Cross-reactivity with BLM pS186 from other sources has not been determined.
Anti-BLM pS186 antibody is useful for ELISA, Immunohistochemistry, and Western Blot. Expect a band approximately ~159kD corresponding to the appropriate cell lysate or extract.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 30% glycerol, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
For research use only.